Increase in negative supercoiling of plasmid DNA in Escherichia coli exposed to cold shock

Tohru Mizushima, Kazuhiro Kataoka, Yasuyuki Ogata, Ryu Ichi Inoue, Kazuhisa Sekimizu

研究成果: Article査読

65 被引用数 (Scopus)

抄録

Negative supercoiling of plasmid DNA in Escherichia coli cells can decrease transiently when exposed to heat shock. The effect of cold shock on DNA supercoiling was examined, and analysis by agarose gel electrophoresis in the presence of chloroquine revealed that negative supercoiling of plasmid DNA in cells increased when cells were exposed to cold shock. This increase was transient and was nil when the cells were pretreated with nalidixic acid, an inhibitor of DNA gyrase. In a mutant deficient in expression of HU protein, the increase in negative supercoiling of DNA by cold shock is less apparent than in wild-type cells. It is proposed that DNA gyrase and HU protein have a role in the DNA supercoiling reaction seen with cold shock.

本文言語English
ページ(範囲)381-386
ページ数6
ジャーナルMolecular Microbiology
23
2
DOI
出版ステータスPublished - 1997

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学

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