抄録
Effects of inhibition of nitric oxide (NO) synthase by N(G)-nitro-L- arginine methyl ester (L-NAME) on the increases in local cerebral blood flow (LCBF) produced in the whisker-to-barrel sensory pathway by vibrissal stimulation were studied in conscious rats with the autoradiographic iodo[14C]antipyrine method. Unilateral whisker stroking increased LCBF in the ipsilateral trigeminal spinal and principal sensory nuclei, contralateral ventral posteromedial thalamic nucleus, and contralateral somatosensory barrel cortex. Intravenous L-NAME (30 mg/kg) lowered baseline LCBF without altering the percent increases due to stimulation. Intracisternal infusions of L-NAME in doses about 10 times the molar content of free arginine in brain inhibited brain NO synthase activity by 88%, but the percent augmentations of LCBF by stimulation remained unchanged. Chronic treatment with L-NAME (50 mg/kg ip twice daily for 4 days) inhibited NO synthase activity in brain by 84% but also failed to reduce the percent increases in LCBF due to stimulation. These results indicate that NO does not mediate the increases in LCBF associated with functional activation.
| 元の言語 | English |
|---|---|
| ジャーナル | American Journal of Physiology - Heart and Circulatory Physiology |
| 巻 | 267 |
| 発行部数 | 6 36-6 |
| 出版物ステータス | Published - 1994 |
| 外部発表 | Yes |
Fingerprint
ASJC Scopus subject areas
- Physiology
- Agricultural and Biological Sciences(all)
これを引用
Increases in local cerebral blood flow associated with somatosensory activation are not mediated by NO. / Adachi, K.; Takahashi, Shinichi; Melzer, P.; Campos, K. L.; Nelson, T.; Kennedy, C.; Sokoloff, L.
：: American Journal of Physiology - Heart and Circulatory Physiology, 巻 267, 番号 6 36-6, 1994.研究成果: Article
}
TY - JOUR
T1 - Increases in local cerebral blood flow associated with somatosensory activation are not mediated by NO
AU - Adachi, K.
AU - Takahashi, Shinichi
AU - Melzer, P.
AU - Campos, K. L.
AU - Nelson, T.
AU - Kennedy, C.
AU - Sokoloff, L.
PY - 1994
Y1 - 1994
N2 - Effects of inhibition of nitric oxide (NO) synthase by N(G)-nitro-L- arginine methyl ester (L-NAME) on the increases in local cerebral blood flow (LCBF) produced in the whisker-to-barrel sensory pathway by vibrissal stimulation were studied in conscious rats with the autoradiographic iodo[14C]antipyrine method. Unilateral whisker stroking increased LCBF in the ipsilateral trigeminal spinal and principal sensory nuclei, contralateral ventral posteromedial thalamic nucleus, and contralateral somatosensory barrel cortex. Intravenous L-NAME (30 mg/kg) lowered baseline LCBF without altering the percent increases due to stimulation. Intracisternal infusions of L-NAME in doses about 10 times the molar content of free arginine in brain inhibited brain NO synthase activity by 88%, but the percent augmentations of LCBF by stimulation remained unchanged. Chronic treatment with L-NAME (50 mg/kg ip twice daily for 4 days) inhibited NO synthase activity in brain by 84% but also failed to reduce the percent increases in LCBF due to stimulation. These results indicate that NO does not mediate the increases in LCBF associated with functional activation.
AB - Effects of inhibition of nitric oxide (NO) synthase by N(G)-nitro-L- arginine methyl ester (L-NAME) on the increases in local cerebral blood flow (LCBF) produced in the whisker-to-barrel sensory pathway by vibrissal stimulation were studied in conscious rats with the autoradiographic iodo[14C]antipyrine method. Unilateral whisker stroking increased LCBF in the ipsilateral trigeminal spinal and principal sensory nuclei, contralateral ventral posteromedial thalamic nucleus, and contralateral somatosensory barrel cortex. Intravenous L-NAME (30 mg/kg) lowered baseline LCBF without altering the percent increases due to stimulation. Intracisternal infusions of L-NAME in doses about 10 times the molar content of free arginine in brain inhibited brain NO synthase activity by 88%, but the percent augmentations of LCBF by stimulation remained unchanged. Chronic treatment with L-NAME (50 mg/kg ip twice daily for 4 days) inhibited NO synthase activity in brain by 84% but also failed to reduce the percent increases in LCBF due to stimulation. These results indicate that NO does not mediate the increases in LCBF associated with functional activation.
KW - brain
KW - functional activation
KW - iodo[C]antipyrine
KW - N(G)-nitro-L-arginine methyl ester
KW - nitric oxide
KW - nitric oxide synthase
KW - whisker barrels
UR - http://www.scopus.com/inward/record.url?scp=0028588492&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028588492&partnerID=8YFLogxK
M3 - Article
C2 - 7528990
AN - SCOPUS:0028588492
VL - 267
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
SN - 0363-6135
IS - 6 36-6
ER -