Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space

Atsushi Sakai, Yoshihiro Murayama, Kei Fujiwara, Takahiro Fujisawa, Saori Sasaki, Satoru Kidoaki, Miho Yanagisawa

研究成果: Article査読

16 被引用数 (Scopus)

抄録

Even though microgels are used in a wide variety of applications, determining their mechanical properties has been elusive because of the difficulties in analysis. In this study, we investigated the surface elasticity of a spherical microgel of gelatin prepared inside a lipid droplet by using micropipet aspiration. We found that gelation inside a microdroplet covered with lipid membranes increased Young's modulus E toward a plateau value E∗ along with a decrease in gel size. In the case of 5.0 wt % gelatin gelled inside a microsized lipid space, the E∗ for small microgels with R ≤ 50 μm was 10-fold higher (35-39 kPa) than that for the bulk gel (∼3 kPa). Structural analysis using circular dichroism spectroscopy and a fluorescence indicator for ordered beta sheets demonstrated that the smaller microgels contained more beta sheets in the structure than the bulk gel. Our finding indicates that the confinement size of gelling polymers becomes a factor in the variation of elasticity of protein-based microgels via secondary structure changes.

本文言語English
ページ(範囲)477-483
ページ数7
ジャーナルACS Central Science
4
4
DOI
出版ステータスPublished - 2018 4 25

ASJC Scopus subject areas

  • 化学 (全般)
  • 化学工学(全般)

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