Indocyanine green fluorescence imaging for evaluation of uterine blood flow in cynomolgus macaque

Iori Kisu, Kouji Banno, Makoto Mihara, Li Yu Lin, Kosuke Tsuji, Megumi Yanokura, Hisako Hara, Jun Araki, Takuya Iida, Takayuki Abe, Keisuke Kouyama, Nobuhiko Suganuma, Daisuke Aoki

研究成果: Article査読

33 被引用数 (Scopus)

抄録

Background: Uterine blood flow is an important factor in uterine viability, but the number of blood vessels required to maintain viability is uncertain. In this study, indocyanine green (ICG) fluorescence imaging was used to examine uterine hemodynamics and vessels associated with uterine blood flow in cynomolgus macaque. Methods: The uterus of a female cynomolgus macaque was cut from the vaginal canal to mimic a situation during trachelectomy or uterine transplantation surgery in which uterine perfusion is maintained only with uterine and ovarian vessels. Intraoperative uterine hemodynamics was observed using ICG fluorescence imaging under conditions in which various nutrient vessels were selected by clamping of blood vessels. A time-intensity curve was plotted using imaging analysis software to measure the T max of uterine perfusion for selected blood vessel patterns. Open surgery was performed with the uterus receiving nutritional support only from uterine vessels on one side. The size of the uterus after surgery was monitored using transabdominal ultrasonography. Results: The resulting time-intensity curves displayed the average intensity in the regions of the uterine corpus and uterine cervix, and in the entire uterus. Analyses of the uterine hemodynamics in the cynomolgus macaque showed that uterine vessels were significantly related to uterine perfusion (P = 0.008), whereas ovarian vessels did not have a significant relationship (P = 0.588). When uterine vessels were clamped, ovarian vessels prolonged the time needed to reach perfusion maximum. Postoperative transabdominal ultrasonography showed that the uterus atrophied over time, without recovery of menstrual discharge. Conclusion: Uterine vessels may be responsible for uterine blood flow, but one uterine vessel may be insufficient to maintain uterine viability in cynomolgus macaque. Our results show that ICG fluorescence imaging is useful for evaluation of uterine blood flow since this method allows real-time observation of uterine hemodynamics.

本文言語English
論文番号e35124
ジャーナルPloS one
7
4
DOI
出版ステータスPublished - 2012 4月 20

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)

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