Induction of apoptosis in mouse brain capillary endothelial cells by cyclosporin A and tacrolimus

Satoko Kochi, Hitomi Takanaga, Hirotami Matsuo, Hisakazu Ohtani, Mikihiko Naito, Takashi Tsuruo, Yasufumi Sawada

研究成果: Article査読

54 被引用数 (Scopus)

抄録

Although cyclosporin A and tacrolimus are used clinically as potent immunosuppressants, there have been reports of neurotoxicity and encephalopathy. A possible mechanism is that these drugs damage the blood- brain barrier (BBB), inducing dysfunction and increased permeability, and are then able to enter the brain. We studied the cytotoxicity of cyclosporin A and tacrolimus, focused on apoptosis induction, using an immortalized cell line established from BALB/c mouse cerebral microvessel endothelial cells (MBEC4). We found that these two drugs induced cell shrinkage, chromatin condensation and DNA fragmentation, which are characteristics of apoptosis. Our data suggest that the induction of apoptosis on the brain capillary endothelial cells may be at least partly involved in the occurrence of immunosuppressant-induced encephalopathy.

本文言語English
ページ(範囲)2255-2260
ページ数6
ジャーナルLife Sciences
66
23
DOI
出版ステータスPublished - 2000 4 28
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 薬理学、毒性学および薬学(全般)

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