Induction of cardiomyocyte differentiation from mouse embryonic stem cells in a confined microfluidic environment

Chen Rei Wan, Seok Chung, Ryo Sudo, Roger D. Kamm

研究成果: Conference contribution

抄録

Embryonic stem cell derived cardiomyocytes are deemed an attractive treatment option for myocardial infarction. Their clinical efficacy, however, has not been unequivocally demonstrated. There is a need for better understanding and characterization of the cardiogenesis process. A microfluidic platform in vitro is used to dissect and better understand the differentiation process. Through this study, we find that while embryoid bodies (EBs) flatten out in a well plate system, differentiated EBs self-assemble into complex 3D structures. The beating regions of EBs are also different. Most beating areas are observed in a ring pattern on 2D well plates around the center, self-assembled beating large 3D aggregates are found in microfluidic devices. Furthermore, inspired by the natural mechanical environment of the heart, we applied uniaxial cyclic mechanical stretch to EBs. Results suggest that prolonged mechanical stimulation acts as a negative regulator of cardiogenesis. From this study, we conclude that the culture environments can influence differentiation of embryonic stem cells into cardiomycytes, and that the use of microfluidic systems can provide new insights into the differentiation process.

本文言語English
ホスト出版物のタイトルProceedings of the ASME Summer Bioengineering Conference 2009, SBC2009
ページ917-918
ページ数2
PART B
DOI
出版ステータスPublished - 2009 12 1
外部発表はい
イベント11th ASME Summer Bioengineering Conference, SBC2009 - Lake Tahoe, CA, United States
継続期間: 2009 6 172009 6 21

出版物シリーズ

名前Proceedings of the ASME Summer Bioengineering Conference 2009, SBC2009
番号PART B

Other

Other11th ASME Summer Bioengineering Conference, SBC2009
国/地域United States
CityLake Tahoe, CA
Period09/6/1709/6/21

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング

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