Induction of cell shape changes through activation of the interleukin-3 common β chain receptor by the RON receptor-type tyrosine kinase

Akihiko Mera, Moritaka Suga, Masayuki Ando, Toshio Suda, Naoto Yamaguchi

研究成果: Article

37 被引用数 (Scopus)

抄録

The RON receptor-type tyrosine kinase, a member of the hepatocyte growth factor receptor family, is a receptor for macrophage-stimulating protein (MSP). Recently, we observed that MSP induces morphological changes in interleukin (IL)-3-dependent Ba/F3 cells ectopically expressing RON. We show here that stimulation of those cells with either MSP or IL-3 increases tyrosine phosphorylation of proteins of 130, 110, 90, 62, and 58 kDa and induces similar morphological changes, accompanied by unique nuclear shape and redistribution of F-actin. A tyrosine kinase inhibitor, genistein, blocked both the increase in tyrosine phosphorylation and morphological changes. Upon stimulation with either MSP or IL-3, prominent tyrosine- phosphorylated pp90 was similarly co-immunoprecipitated with the common β chain of IL-3 receptor (β(c)). Unlike IL-3, stimulation with MSP increased tyrosine phosphorylation of β(c) without activation of JAK2, resulting in morphological changes with modest cell growth. Confocal immunofluorescence analyses showed colocalization of RON, β(c), and tyrosine-phosphorylated proteins. In vitro kinase assays revealed that autophosphorylated RON phosphorylated β(c). These results suggest that the signaling pathway for morphological changes through β(c) and its associated protein pp90 is distinct from the pathway for cell growth in the IL-3 signal transduction system.

本文言語English
ページ(範囲)15766-15774
ページ数9
ジャーナルJournal of Biological Chemistry
274
22
DOI
出版ステータスPublished - 1999 5 28

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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