Induction of Fas-mediated apoptosis in p53-transfected human colon carcinoma cells

T. Tamura, N. Aoyama, H. Saya, H. Haga, S. Futami, M. Miyamoto, T. Koh, T. Ariyasu, M. Tachi, M. Kasuga, R. Takahashi

研究成果: Article

94 引用 (Scopus)


To investigate the biological function of p53 in colon carcinoma cells, a wild-type p53 expression plasmid under the control of the human cytomegalovirus promoter was stably transfected into the human colon adenocarcinoma cell line WiDr, which carries a mutation of the p53 gene at codon 273. Exogenous wild-type p53 transcripts were detected at various expression levels in 8 of 117 G418-resistant clones. The growth rates of the wild-type p53+ clones in culture did not change significantly. The efficiency of colony formation in soft agar, however, was completely suppressed in two wild-type p53+ clones. This is the first to demonstrate the feasibility of stable transfection of the wild-type p53 gene under the control of non-inducible promoter in human colon cancer cells. The major alteration found was that wild-type p53+ cells which were incubated with anti-Fas IgM showed marked cytolysis with preferential overexpression of wild-type p53 accompanied by overexpression of a cyclin-dependent kinase inhibitor, WAF1, whereas the endogenous mutant p53 retained its expression level. The findings suggest that a Fas-initiated pathway is incidentally linked to a p53-dependent apoptotic pathway through the reconstituted wild-type p53 gene in WiDr cells. This model should help elucidating the additional role of the p53 tumor suppressor gene and the mechanism of apoptosis in colon carcinoma cells.

出版物ステータスPublished - 1995 1 1


ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research


Tamura, T., Aoyama, N., Saya, H., Haga, H., Futami, S., Miyamoto, M., Koh, T., Ariyasu, T., Tachi, M., Kasuga, M., & Takahashi, R. (1995). Induction of Fas-mediated apoptosis in p53-transfected human colon carcinoma cells. Oncogene, 11(10), 1939-1946.