Induction of superficial cortical layer neurons from mouse embryonic stem cells by valproic acid

Berry Juliandi, Masahiko Abematsu, Tsukasa Sanosaka, Keita Tsujimura, Austin Smith, Kinichi Nakashima

研究成果: Article査読

36 被引用数 (Scopus)

抄録

Within the developing mammalian cortex, neural progenitors first generate deep-layer neurons and subsequently more superficial-layer neurons, in an inside-out manner. It has been reported recently that mouse embryonic stem cells (mESCs) can, to some extent, recapitulate cortical development in vitro, with the sequential appearance of neurogenesis markers resembling that in the developing cortex. However, mESCs can only recapitulate early corticogenesis; superficial-layer neurons, which are normally produced in later developmental periods in vivo, are under-represented. This failure of mESCs to reproduce later corticogenesis in vitro implies the existence of crucial factor(s) that are absent or uninduced in existing culture systems. Here we show that mESCs can give rise to superficial-layer neurons efficiently when treated with valproic acid (VPA), a histone deacetylase inhibitor. VPA treatment increased the production of Cux1-positive superficial-layer neurons, and decreased that of Ctip2-positive deep-layer neurons. These results shed new light on the mechanisms of later corticogenesis.

本文言語English
ページ(範囲)23-31
ページ数9
ジャーナルNeuroscience Research
72
1
DOI
出版ステータスPublished - 2012 1月
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)

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