Induction of Th17 cells by segmented filamentous bacteria in the murine intestine

Adam M. Farkas, Casandra Panea, Yoshiyuki Goto, Gaku Nakato, Marta Galan-Diez, Seiko Narushima, Kenya Honda, Ivaylo I. Ivanov

研究成果: Article査読

42 被引用数 (Scopus)

抄録

Segmented filamentous bacteria (SFB) are Gram-positive, anaerobic, spore-forming commensals that reside in the gut of many animal species. Described more than forty years ago, SFB have recently gained interest due to their unique ability to modulate the host immune system through induction of IgA and Th17 cells. Here, we describe a collection of methods to detect and quantify SFB and SFB adhesion in intestinal mucosa, as well as SFB-specific CD4 T cells in the lamina propria. In addition, we describe methods for purification of SFB from fecal material of SFB-monoassociated gnotobiotic mice. Using these methods we examine the kinetics of SFB colonization and Th17 cell induction. We also show that SFB colonize unevenly the intestinal mucosa and that SFB adherence occurs predominantly in the terminal ileum and correlates with an increased proportion of SFB-specific Th17 cells.

本文言語English
ページ(範囲)104-111
ページ数8
ジャーナルJournal of Immunological Methods
421
DOI
出版ステータスPublished - 2015 5 29

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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