Inefficient Transcription of the Myelin Basic Protein Gene Possibly Causes Hypomyelination in Myelin‐Deficient Mutant Mice

Hideyuki Okano, Masayuki Miura, Akira Moriguchi, Kazuhiro Ikenaka, Yasuzo Tsukada, Katsuhiko Mikoshiba

研究成果: Article査読

37 被引用数 (Scopus)

抄録

Abstract: A hereditary dysmyelination mutation, named myelin deficient (mld), is considered to be allelic to shiverer, a deletion mutation of the myelin basic protein (MBP) gene. The present study showed that MBP expression is greatly reduced in mld, but that it is still detectable. Northern blot analysis revealed that the pronounced decrease in the MBP level in mld resulted from a reduced mRNA level and was not caused by deletion of a large portion of the MBP gene as in shiverer. Southern blot studies with BamHI‐digested chromosomal DNA suggested some part of the MBP gene, at least the 5′‐portion, was duplicated in mld. These results indicated that the mld and shiverer mutations were different from each other, even though genetic allelism between the two was reconfirmed. We also examined the developmental pattern of the gene expression of MBP and that of another protein, myelin proteolipid protein (PLP), specifically expressed in the oligodendrocyte, in mld by RNA dot blot study. The mRNA level of MBP in mld was greatly reduced during the active myelination stages, gradually increasing and remaining constant in the later stages. The PLP‐mRNA content in mld was almost normal (60–80% that of control) at any stage of development. All these findings imply that the primary defect in mld is due to reduced transcriptional activity of the MBP gene.

本文言語English
ページ(範囲)470-476
ページ数7
ジャーナルJournal of Neurochemistry
48
2
DOI
出版ステータスPublished - 1987 2
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 細胞および分子神経科学

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