Influence of Mn Ion the Action of Dibutyryl Cyclic AMP and Forskolin on Contraction, Membrane Response, and Cyclic AMP-Dependent Protein Kinase Activity in Rat Myometrium

Hiroyoshi Inoue, Takuro Osa, Masayuki Okuda

研究成果: Article

3 引用 (Scopus)

抄録

Contractile response, membrane activity, and protein kinase A (PKA) activity were measured on the longitudinal muscle taken from the estrogen-treated rat uterus, and the influence of Mn ion on the inhibitory effects caused by db cAMP and forskolin was investigated. Phasic contractions generated in the muscle taken from the middle portion of uterus were depressed to 48 and 83% by 30μM db cAMP and 0.1μM forskolin in Mg-free Krebs solution, respectively; phasic contractions were more strongly depressed by the agents in the solution containing 0.2 mM Mn. Action potentials consisted of spike and plateau components, and the duration of the plateau potential was reduced by the application of the agents; membrane activity was more strongly depressed in the presence of 0.2 mM Mn. The contractile depression caused by db cAMP was reduced and by forskolin was enhanced by pretreatment of the tissue with 0.6 mM Mn for 30min. The PKA activity was increased by 39 and 6% of the control, when 30μM db cAMP and 0.1μM forskolin were applied, respectively; the PKA activity in response to db cAMP and forskolin was reduced and enhanced, respectively, when the tissues were pretreated with 0.6 mM Mn. It was proposed that Mn ions permeated into cell interior when the muscle was exposed to 0.6 mM Mn, so that the effects of the agents were differently affected. It was also shown that plateau potential dominated in the muscle taken from the ovarian portion, and the contractile inhibition caused by the agents was far weaker.

元の言語English
ページ(範囲)455-472
ページ数18
ジャーナルJapanese Journal of Physiology
43
発行部数4
DOI
出版物ステータスPublished - 1993 1 1
外部発表Yes

Fingerprint

Bucladesine
Myometrium
Colforsin
Cyclic AMP-Dependent Protein Kinases
Ions
Membranes
Muscles
Uterus
Action Potentials
Estrogens
Membrane Proteins

ASJC Scopus subject areas

  • Physiology

これを引用

@article{aad5111590054ccc99564ac2765aae4c,
title = "Influence of Mn Ion the Action of Dibutyryl Cyclic AMP and Forskolin on Contraction, Membrane Response, and Cyclic AMP-Dependent Protein Kinase Activity in Rat Myometrium",
abstract = "Contractile response, membrane activity, and protein kinase A (PKA) activity were measured on the longitudinal muscle taken from the estrogen-treated rat uterus, and the influence of Mn ion on the inhibitory effects caused by db cAMP and forskolin was investigated. Phasic contractions generated in the muscle taken from the middle portion of uterus were depressed to 48 and 83{\%} by 30μM db cAMP and 0.1μM forskolin in Mg-free Krebs solution, respectively; phasic contractions were more strongly depressed by the agents in the solution containing 0.2 mM Mn. Action potentials consisted of spike and plateau components, and the duration of the plateau potential was reduced by the application of the agents; membrane activity was more strongly depressed in the presence of 0.2 mM Mn. The contractile depression caused by db cAMP was reduced and by forskolin was enhanced by pretreatment of the tissue with 0.6 mM Mn for 30min. The PKA activity was increased by 39 and 6{\%} of the control, when 30μM db cAMP and 0.1μM forskolin were applied, respectively; the PKA activity in response to db cAMP and forskolin was reduced and enhanced, respectively, when the tissues were pretreated with 0.6 mM Mn. It was proposed that Mn ions permeated into cell interior when the muscle was exposed to 0.6 mM Mn, so that the effects of the agents were differently affected. It was also shown that plateau potential dominated in the muscle taken from the ovarian portion, and the contractile inhibition caused by the agents was far weaker.",
keywords = "db cAMP, forskolin, Mn, myometrium, protein kinase A",
author = "Hiroyoshi Inoue and Takuro Osa and Masayuki Okuda",
year = "1993",
month = "1",
day = "1",
doi = "10.2170/jjphysiol.43.455",
language = "English",
volume = "43",
pages = "455--472",
journal = "Journal of Physiological Sciences",
issn = "1880-6546",
publisher = "Springer Japan",
number = "4",

}

TY - JOUR

T1 - Influence of Mn Ion the Action of Dibutyryl Cyclic AMP and Forskolin on Contraction, Membrane Response, and Cyclic AMP-Dependent Protein Kinase Activity in Rat Myometrium

AU - Inoue, Hiroyoshi

AU - Osa, Takuro

AU - Okuda, Masayuki

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Contractile response, membrane activity, and protein kinase A (PKA) activity were measured on the longitudinal muscle taken from the estrogen-treated rat uterus, and the influence of Mn ion on the inhibitory effects caused by db cAMP and forskolin was investigated. Phasic contractions generated in the muscle taken from the middle portion of uterus were depressed to 48 and 83% by 30μM db cAMP and 0.1μM forskolin in Mg-free Krebs solution, respectively; phasic contractions were more strongly depressed by the agents in the solution containing 0.2 mM Mn. Action potentials consisted of spike and plateau components, and the duration of the plateau potential was reduced by the application of the agents; membrane activity was more strongly depressed in the presence of 0.2 mM Mn. The contractile depression caused by db cAMP was reduced and by forskolin was enhanced by pretreatment of the tissue with 0.6 mM Mn for 30min. The PKA activity was increased by 39 and 6% of the control, when 30μM db cAMP and 0.1μM forskolin were applied, respectively; the PKA activity in response to db cAMP and forskolin was reduced and enhanced, respectively, when the tissues were pretreated with 0.6 mM Mn. It was proposed that Mn ions permeated into cell interior when the muscle was exposed to 0.6 mM Mn, so that the effects of the agents were differently affected. It was also shown that plateau potential dominated in the muscle taken from the ovarian portion, and the contractile inhibition caused by the agents was far weaker.

AB - Contractile response, membrane activity, and protein kinase A (PKA) activity were measured on the longitudinal muscle taken from the estrogen-treated rat uterus, and the influence of Mn ion on the inhibitory effects caused by db cAMP and forskolin was investigated. Phasic contractions generated in the muscle taken from the middle portion of uterus were depressed to 48 and 83% by 30μM db cAMP and 0.1μM forskolin in Mg-free Krebs solution, respectively; phasic contractions were more strongly depressed by the agents in the solution containing 0.2 mM Mn. Action potentials consisted of spike and plateau components, and the duration of the plateau potential was reduced by the application of the agents; membrane activity was more strongly depressed in the presence of 0.2 mM Mn. The contractile depression caused by db cAMP was reduced and by forskolin was enhanced by pretreatment of the tissue with 0.6 mM Mn for 30min. The PKA activity was increased by 39 and 6% of the control, when 30μM db cAMP and 0.1μM forskolin were applied, respectively; the PKA activity in response to db cAMP and forskolin was reduced and enhanced, respectively, when the tissues were pretreated with 0.6 mM Mn. It was proposed that Mn ions permeated into cell interior when the muscle was exposed to 0.6 mM Mn, so that the effects of the agents were differently affected. It was also shown that plateau potential dominated in the muscle taken from the ovarian portion, and the contractile inhibition caused by the agents was far weaker.

KW - db cAMP

KW - forskolin

KW - Mn

KW - myometrium

KW - protein kinase A

UR - http://www.scopus.com/inward/record.url?scp=0027422530&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027422530&partnerID=8YFLogxK

U2 - 10.2170/jjphysiol.43.455

DO - 10.2170/jjphysiol.43.455

M3 - Article

C2 - 8114357

AN - SCOPUS:0027422530

VL - 43

SP - 455

EP - 472

JO - Journal of Physiological Sciences

JF - Journal of Physiological Sciences

SN - 1880-6546

IS - 4

ER -