Influence of sugar chain on fibrin affinity of recombinant t-PA

Shoichi Aoki, Norihide Shimizu, Manabu Shimonishi, Masaru Kitagawa, Kazuo Okumura, Yusuke Tanigawara

研究成果: Article査読

3 被引用数 (Scopus)

抄録

The role of the sugar chain on the fibrin affinity property of tissue plasminogen activator (t-PA) was investigated using two variants of wild type t-PA (WT t-PA I and WT t-PA II) and mutant type t-PA (mt-PA ; Gln117 t-PA I and Gln117 t-PA II), whose sugar chains have different structures. In terms of fibrin affinity, Gln117 t-PA was higher than WT t-PA ; moreover, Type II was higher than Type I. Bindings mediated via finger domain (F mode) and kringle 2 domain (K2 mode) were distinguished using ε-amino caproic acid (EACA). Consequently, F mode and K2 mode bindings were inhibited by the sugar chains at Asn117 and 184, respectively. These results were assumed to be due to the steric hindrance of the sugar chains.

本文言語English
ページ(範囲)295-298
ページ数4
ジャーナルBiological and Pharmaceutical Bulletin
24
3
DOI
出版ステータスPublished - 2001

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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