Influence of temperature on the myocardial cells death by an extracellular talaporfin sodium-induced photosensitization reaction

Emiyu Ogawa, Hiromi Takenoya, Tsunenori Arai

研究成果: Conference contribution

抄録

We have proposed to apply the photosensitization reaction in myocardium interstitial fluid using talaporfin sodium to realize less-heated electrical conduction block for a tachyarrhythmia treatment: PD Ablation®. The cytotoxicity of the extracellular photosensitization reaction efficiency may change by the talaporfin sodium binding with serum proteins. These binding would change with solution temperature. We investigated the binding behavior of talaporfin sodium with human serum albumin (HSA), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) changing solution temperature from 17 to 37°C. We also studied the photocytotoxicity change by solution temperature of 17 and 37°C measuring cell lethality by WST assay using fetal bovine serum. The binding ratio of talaporfin sodium with HDL and LDL decreased 6.3% and 12.8% with temperature increasing from 17 to 37°C. There was no significant difference in the case of HSA. The cell lethality was increased about 30% with temperature increasing from 17 to 37°C. The myocardium tissue temperature increase was reported that less than 5°C in the case of our PD Ablation®. We think that the photocytotoxicity change by these temperature increasing would be negligible in our PD Ablation®. We suggest that the temperature maintaining would be necessary to keep the photocytotoxicity efficiency in the case of the open surgery that would cause the tissue surface temperature decreasing.

本文言語English
ホスト出版物のタイトルOptical Interactions with Tissue and Cells XXVII
編集者E. Duco Jansen
出版社SPIE
ISBN(電子版)9781628419405
DOI
出版ステータスPublished - 2016
イベントOptical Interactions with Tissue and Cells XXVII - San Francisco, United States
継続期間: 2016 2月 142016 2月 17

出版物シリーズ

名前Progress in Biomedical Optics and Imaging - Proceedings of SPIE
9706
ISSN(印刷版)1605-7422

Other

OtherOptical Interactions with Tissue and Cells XXVII
国/地域United States
CitySan Francisco
Period16/2/1416/2/17

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 生体材料
  • 原子分子物理学および光学
  • 放射線学、核医学およびイメージング

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