Inhibition of heat-shock protein 27 reduces 5-Fluorouracil-acquired resistance in human colon cancer cells

Yusuke Asada, Masashi Tsuruta, Koji Okabayashi, Kohei Shigeta, Takashi Ishida, Takehiro Shimada, Hirofumi Suzumura, Kaoru Koishikawa, Shingo Akimoto, Hirotoshi Hasegawa, Yuko Kitagawa

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Background/Aim: In previous work we showed that expression of heat-shock protein 27 (HSP27; encoded by HSPB1) was associated with inherent resistance to 5-fluorouracil (5-FU). However, the relationship between HSP27 and acquired resistance remains unknown. Materials and Methods: We generated an acquired resistance model (WiDr-R) of a colon cancer cell line by exposing WiDr cells to 5-FU. Cell viability assays under treatment with 5-FU, as well as down-regulation of HSP27 using small interfering HSP27 RNA, were performed. HSP27 mRNA and protein expression was analyzed using real-time polymerase chain reaction and western blotting. Results: 5-FU-acquired resistance induced overexpression of HSP27 mRNA and protein levels in WiDr-R cells. Furthermore, siRNA knockdown of HSP27 in WiDr-R cells reduced 5-FU-acquired resistance. Conclusion: These findings demonstrate that HSP27 is associated with 5-FU resistance in human colon cancer cell cells and suggest that HSP27 regulation represents a novel approach to overcoming chemoresistance in colorectal cancer.

本文言語English
ページ(範囲)1283-1290
ページ数8
ジャーナルAnticancer research
41
3
DOI
出版ステータスPublished - 2021 3

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

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