TY - CONF
T1 - Inhibition of influenza virus infection by minimized peptides which bind to hemagglutinin
AU - Saito, Tomomi
AU - Matsubara, Teruhiko
AU - Sato, Toshinori
PY - 2006/10/19
Y1 - 2006/10/19
N2 - Influenza virus hemagglutinin (HA) binds to sialylgalactose (Neu5Ac-Gal) structure on host cells at the first step of infection. The peptide drugs are able to block the virus-sugar interaction. In our previous study, the HA-binding pentadecapeptide has been obtained from a random phage-displayed peptide library. However, it is considered that all amino acid residues are not involved in the interaction with HA, because the size of sugarbinding pocket in HA is too small to fill with pentadecapeptide. In order to identify the active residues, several peptide fragments were prepared. Plaque assay indicated that a N-terminal fragment had a high inhibition activity for influenza virus infection than the corresponding original peptide. We finally identified minimized HA-binding peptide. Furthermore, we showed that stearyl peptide was suitable for aggregate formation that was essential for inhibition of influenza virus infection.
AB - Influenza virus hemagglutinin (HA) binds to sialylgalactose (Neu5Ac-Gal) structure on host cells at the first step of infection. The peptide drugs are able to block the virus-sugar interaction. In our previous study, the HA-binding pentadecapeptide has been obtained from a random phage-displayed peptide library. However, it is considered that all amino acid residues are not involved in the interaction with HA, because the size of sugarbinding pocket in HA is too small to fill with pentadecapeptide. In order to identify the active residues, several peptide fragments were prepared. Plaque assay indicated that a N-terminal fragment had a high inhibition activity for influenza virus infection than the corresponding original peptide. We finally identified minimized HA-binding peptide. Furthermore, we showed that stearyl peptide was suitable for aggregate formation that was essential for inhibition of influenza virus infection.
KW - Hemagglutinin
KW - Influenza virus
KW - Peptide
KW - Phage display method
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M3 - Paper
AN - SCOPUS:33749873647
T2 - 55th SPSJ Annual Meeting
Y2 - 24 May 2006 through 26 May 2006
ER -