Inhibitory effect of oxycoumarins isolated from the Thai medicinal plant Clausena guillauminii on the inflammation mediators, iNOS, TNF-α, and COX-2 expression in mouse macrophage RAW 264.7

Tomonori Nakamura, Naoko Kodama, Yu Arai, Takuya Kumamoto, Yoshihiro Higuchi, Chaiyo Chaichantipyuth, Tsutomu Ishikawa, Koichi Ueno, Shingo Yano

研究成果: Article

60 引用 (Scopus)

抄録

In the present study, we investigated the inhibitory effect of the known oxycoumarins poncitrin (3), osthol (4), and xanthoxyletin (5), newly isolated from Clausena guillauminii (Rutaceae), together with the known carbazoles heptaphylline (1) and 7-methoxyheptaphylline (2) on inducible-nitric oxide synthase (iNOS) expression induced by lipopolysaccharide (LPS) and the NO generation in RAW 264.7 mouse macrophages. Isolation of active oxycoumarins was guided by Western blot analysis of iNOS protein expression. These oxycoumarins showed an inhibitory effect on iNOS protein expression at 10 μM. Further examination of the inhibitory effects of these compounds on inflammation mediators revealed that the synthesis of nitric oxide (NO) and the protein expression of tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2) were inhibited by 5. It was expected that these compounds show anti-inflammatory activities.

元の言語English
ページ(範囲)21-27
ページ数7
ジャーナルJournal of Natural Medicines
63
発行部数1
DOI
出版物ステータスPublished - 2009 1
外部発表Yes

Fingerprint

Clausena
Inflammation Mediators
Macrophages
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Medicinal Plants
Tumor Necrosis Factor-alpha
Nitric Oxide
Carbazoles
Rutaceae
Proteins
Lipopolysaccharides
Anti-Inflammatory Agents
Western Blotting

ASJC Scopus subject areas

  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Complementary and alternative medicine

これを引用

Inhibitory effect of oxycoumarins isolated from the Thai medicinal plant Clausena guillauminii on the inflammation mediators, iNOS, TNF-α, and COX-2 expression in mouse macrophage RAW 264.7. / Nakamura, Tomonori; Kodama, Naoko; Arai, Yu; Kumamoto, Takuya; Higuchi, Yoshihiro; Chaichantipyuth, Chaiyo; Ishikawa, Tsutomu; Ueno, Koichi; Yano, Shingo.

:: Journal of Natural Medicines, 巻 63, 番号 1, 01.2009, p. 21-27.

研究成果: Article

Nakamura, Tomonori ; Kodama, Naoko ; Arai, Yu ; Kumamoto, Takuya ; Higuchi, Yoshihiro ; Chaichantipyuth, Chaiyo ; Ishikawa, Tsutomu ; Ueno, Koichi ; Yano, Shingo. / Inhibitory effect of oxycoumarins isolated from the Thai medicinal plant Clausena guillauminii on the inflammation mediators, iNOS, TNF-α, and COX-2 expression in mouse macrophage RAW 264.7. :: Journal of Natural Medicines. 2009 ; 巻 63, 番号 1. pp. 21-27.
@article{1be216b1c1ae409cb4ea40f86d5a6752,
title = "Inhibitory effect of oxycoumarins isolated from the Thai medicinal plant Clausena guillauminii on the inflammation mediators, iNOS, TNF-α, and COX-2 expression in mouse macrophage RAW 264.7",
abstract = "In the present study, we investigated the inhibitory effect of the known oxycoumarins poncitrin (3), osthol (4), and xanthoxyletin (5), newly isolated from Clausena guillauminii (Rutaceae), together with the known carbazoles heptaphylline (1) and 7-methoxyheptaphylline (2) on inducible-nitric oxide synthase (iNOS) expression induced by lipopolysaccharide (LPS) and the NO generation in RAW 264.7 mouse macrophages. Isolation of active oxycoumarins was guided by Western blot analysis of iNOS protein expression. These oxycoumarins showed an inhibitory effect on iNOS protein expression at 10 μM. Further examination of the inhibitory effects of these compounds on inflammation mediators revealed that the synthesis of nitric oxide (NO) and the protein expression of tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2) were inhibited by 5. It was expected that these compounds show anti-inflammatory activities.",
keywords = "Clausena guillauminii, iNOS, Oxycoumarin, RAW 264.7, Rutaceae, Xanthoxyletin",
author = "Tomonori Nakamura and Naoko Kodama and Yu Arai and Takuya Kumamoto and Yoshihiro Higuchi and Chaiyo Chaichantipyuth and Tsutomu Ishikawa and Koichi Ueno and Shingo Yano",
year = "2009",
month = "1",
doi = "10.1007/s11418-008-0277-5",
language = "English",
volume = "63",
pages = "21--27",
journal = "Journal of Natural Medicines",
issn = "1861-0293",
publisher = "Springer Japan",
number = "1",

}

TY - JOUR

T1 - Inhibitory effect of oxycoumarins isolated from the Thai medicinal plant Clausena guillauminii on the inflammation mediators, iNOS, TNF-α, and COX-2 expression in mouse macrophage RAW 264.7

AU - Nakamura, Tomonori

AU - Kodama, Naoko

AU - Arai, Yu

AU - Kumamoto, Takuya

AU - Higuchi, Yoshihiro

AU - Chaichantipyuth, Chaiyo

AU - Ishikawa, Tsutomu

AU - Ueno, Koichi

AU - Yano, Shingo

PY - 2009/1

Y1 - 2009/1

N2 - In the present study, we investigated the inhibitory effect of the known oxycoumarins poncitrin (3), osthol (4), and xanthoxyletin (5), newly isolated from Clausena guillauminii (Rutaceae), together with the known carbazoles heptaphylline (1) and 7-methoxyheptaphylline (2) on inducible-nitric oxide synthase (iNOS) expression induced by lipopolysaccharide (LPS) and the NO generation in RAW 264.7 mouse macrophages. Isolation of active oxycoumarins was guided by Western blot analysis of iNOS protein expression. These oxycoumarins showed an inhibitory effect on iNOS protein expression at 10 μM. Further examination of the inhibitory effects of these compounds on inflammation mediators revealed that the synthesis of nitric oxide (NO) and the protein expression of tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2) were inhibited by 5. It was expected that these compounds show anti-inflammatory activities.

AB - In the present study, we investigated the inhibitory effect of the known oxycoumarins poncitrin (3), osthol (4), and xanthoxyletin (5), newly isolated from Clausena guillauminii (Rutaceae), together with the known carbazoles heptaphylline (1) and 7-methoxyheptaphylline (2) on inducible-nitric oxide synthase (iNOS) expression induced by lipopolysaccharide (LPS) and the NO generation in RAW 264.7 mouse macrophages. Isolation of active oxycoumarins was guided by Western blot analysis of iNOS protein expression. These oxycoumarins showed an inhibitory effect on iNOS protein expression at 10 μM. Further examination of the inhibitory effects of these compounds on inflammation mediators revealed that the synthesis of nitric oxide (NO) and the protein expression of tumor necrosis factor-α (TNF-α) and cyclooxygenase-2 (COX-2) were inhibited by 5. It was expected that these compounds show anti-inflammatory activities.

KW - Clausena guillauminii

KW - iNOS

KW - Oxycoumarin

KW - RAW 264.7

KW - Rutaceae

KW - Xanthoxyletin

UR - http://www.scopus.com/inward/record.url?scp=57049104672&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57049104672&partnerID=8YFLogxK

U2 - 10.1007/s11418-008-0277-5

DO - 10.1007/s11418-008-0277-5

M3 - Article

C2 - 18636311

AN - SCOPUS:57049104672

VL - 63

SP - 21

EP - 27

JO - Journal of Natural Medicines

JF - Journal of Natural Medicines

SN - 1861-0293

IS - 1

ER -