Insertion of foreign random sequences of 120 amino acid residues into an active enzyme

Nobuhide Doi, Mitsuhiro Itaya, Tetsuya Yomo, Seiichi Tokura, Hiroshi Yanagawa

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Random sequences of 120-130 amino acid residues were inserted into a surface loop region of Escherichia coli RNase HI. This library was screened and about 10% of the clones were found to retain RNase H activity. Subsequent random mutagenesis led to an increase in RNase H activity and solubility of the protein. The inserted regions mere found not to contribute to the secondary structure of the mutant protein. The high frequency of insertion of flexible sequences and the increase in the protein's function by further mutagenesis simulate one of the events in protein evolution.

本文言語English
ページ(範囲)177-180
ページ数4
ジャーナルFEBS Letters
402
2-3
DOI
出版ステータスPublished - 1997 1月 27
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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