Insertion of foreign random sequences of 120 amino acid residues into an active enzyme

Nobuhide Doi, Mitsuhiro Itaya, Tetsuya Yomo, Seiichi Tokura, Hiroshi Yanagawa

研究成果: Article

24 引用 (Scopus)

抜粋

Random sequences of 120-130 amino acid residues were inserted into a surface loop region of Escherichia coli RNase HI. This library was screened and about 10% of the clones were found to retain RNase H activity. Subsequent random mutagenesis led to an increase in RNase H activity and solubility of the protein. The inserted regions mere found not to contribute to the secondary structure of the mutant protein. The high frequency of insertion of flexible sequences and the increase in the protein's function by further mutagenesis simulate one of the events in protein evolution.

元の言語English
ページ(範囲)177-180
ページ数4
ジャーナルFEBS Letters
402
発行部数2-3
DOI
出版物ステータスPublished - 1997 1 27

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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