Insertion of unmarked DNA sequences in multiple loci of the bacillus subtilis 168 genome: An efficient selection method

Rie Uotsu-Tomita, Shinya Kaneko, Kenji Tsuge, Mitsuhiro Itaya

研究成果: Article査読

7 被引用数 (Scopus)

抄録

The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.

本文言語English
ページ(範囲)1036-1039
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
69
5
DOI
出版ステータスPublished - 2005 5

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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