Insertional gene fusion technology

Nobuhide Doi, Hiroshi Yanagawa

研究成果: Article

46 引用 (Scopus)

抜粋

The classical 'end to end' gene fusion technique has widely been used for monitoring gene expression, biological screening and purification of recombinant proteins. Recent progress with the 'insertional' gene fusion approach, on the other hand, has demonstrated that this technique can be utilized for membrane protein topology analysis, display of randomized protein libraries and design of biosensor proteins. In this review, we describe examples of insertional gene fusion and compare the old and new gene fusion techniques. Copyright (C) 1999 Federation of European Biochemical Societies.

元の言語English
ページ(範囲)1-4
ページ数4
ジャーナルFEBS Letters
457
発行部数1
DOI
出版物ステータスPublished - 1999 8 20
外部発表Yes

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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