Integrity of plasmid deoxyribonucleic acid after application of laser-induced stress waves used for gene transfection

Mitsuhiro Terakawa; Shunichi Sato; Hiroshi Ashida; Hideyuki Okano; Minoru Obara

doi:10.1143/JJAP.45.L768

Integrity of plasmid deoxyribonucleic acid after application of laser-induced stress waves used for gene transfection

Mitsuhiro Terakawa, Shunichi Sato, Hiroshi Ashida, Hideyuki Okano, Minoru Obara

研究成果: Article › 査読

2 被引用数 (Scopus)

抄録

We investigated the effects of laser-induced stress waves (LISWs) on the integrity of plasmid deoxyribonucleic acid (DNA). Plasmid DNA buffer was exposed to LISWs generated by the irradiation of a solid target with nanosecond laser pulses. Electrophoresis assessment revealed that the plasmid DNA was not affected by the application of LISWs under the conditions used for our previous gene transfection experiments, suggesting a low invasiveness of LISW-mediated gene transfection.

本文言語	English
ページ（範囲）	L768-L769
ジャーナル	Japanese Journal of Applied Physics, Part 2: Letters
巻	45
号	29-32
DOI	https://doi.org/10.1143/JJAP.45.L768
出版ステータス	Published - 2006 8月 11

ASJC Scopus subject areas

工学（全般）
物理学および天文学（全般）

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10.1143/JJAP.45.L768

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abstract = "We investigated the effects of laser-induced stress waves (LISWs) on the integrity of plasmid deoxyribonucleic acid (DNA). Plasmid DNA buffer was exposed to LISWs generated by the irradiation of a solid target with nanosecond laser pulses. Electrophoresis assessment revealed that the plasmid DNA was not affected by the application of LISWs under the conditions used for our previous gene transfection experiments, suggesting a low invasiveness of LISW-mediated gene transfection.",

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author = "Mitsuhiro Terakawa and Shunichi Sato and Hiroshi Ashida and Hideyuki Okano and Minoru Obara",

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AU - Terakawa, Mitsuhiro

AU - Sato, Shunichi

AU - Ashida, Hiroshi

AU - Okano, Hideyuki

AU - Obara, Minoru

PY - 2006/8/11

Y1 - 2006/8/11

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AB - We investigated the effects of laser-induced stress waves (LISWs) on the integrity of plasmid deoxyribonucleic acid (DNA). Plasmid DNA buffer was exposed to LISWs generated by the irradiation of a solid target with nanosecond laser pulses. Electrophoresis assessment revealed that the plasmid DNA was not affected by the application of LISWs under the conditions used for our previous gene transfection experiments, suggesting a low invasiveness of LISW-mediated gene transfection.

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KW - Electrophoresis

KW - Gene therapy

KW - Gene transfection

KW - Heating

KW - Laser-induced stress waves

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JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes

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Integrity of plasmid deoxyribonucleic acid after application of laser-induced stress waves used for gene transfection

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