Interkinetic and migratory behavior of a cohort of neocortical neurons arising in the early embryonic murine cerebral wall

Takao Takahashi, Richard S. Nowakowski, Verne S. Caviness

研究成果: Article査読

89 被引用数 (Scopus)

抄録

Neocortical neuronogenesis occurs in the pseudostratified ventricular epithelium (PVE) where nuclei of proliferative cells undergo interkinetic nuclear movement. A fraction of daughter cells exits the cell cycle as neurons (the quiescent, or Q, fraction), whereas a complementary fraction remains in the cell cycle (the proliferative, or P, fraction). By means of sequential thymidine and bromodeoxyuridine injections in mouse on embryonic day 14, we have monitored the proliferative and postmitotic migratory behaviors of 1 and 2 hr cohorts of PVE cells defined by the injection protocols. Soon after mitosis, the Q fraction partitions into a rapidly exiting (up to 50 μm/hr) subpopulation (Q(r)) and a more slowly exiting (6 μm/hr) subpopulation (Q(s)). Q(r) and Q(s) are separated as two distributions on exit from the ventricular zone with an interpeak distance of ~ 40 μm. Cells in Q(r) and Q(s) migrate through the intermediate zone with no significant change in the interpeak distance, suggesting that they migrate at approximately the same velocities. The rate of migration increases with ascent through the intermediate zone (average 2-6.4 μm/hr) slowing only transiently on entry into the developing cortex. Within the cortex, Q(r) and Q(s) merge to form a single distribution most concentrated over layer V.

本文言語English
ページ(範囲)5762-5776
ページ数15
ジャーナルJournal of Neuroscience
16
18
DOI
出版ステータスPublished - 1996 9 15
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)

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