Interleukin 4 downregulates cell growth and prostaglandin release of human mesangial cells

Yuichi Nakazato, Hirokazu Okada, Atsuhisa Sato, Yasushi Iwaita, Tomoko Hayashida, Matsuhiko Hayashi, Hiromichi Suzuki, Takao Saruta

研究成果: Article査読

16 被引用数 (Scopus)

抄録

Several clinical studies have proposed that a T cell derived cytokine IL-4 is operative in glomerulonephritis; however, its biological activities on renal cells have not been investigated. To elucidate its possible role in glomerulonephritis, we have examined whether IL-4 has effect on cell growth and prostaglandins synthesis using cultured mesangial cells (MC). IL-4 dose-dependently suppressed DNA synthesis and cell proliferation. IL-4 (10 ng/ml) alone did not affect prostaglandin E2 (PGE2) synthesis by mesangial cells, though, it inhibited IL-1α- and TNFα-stimulated PGE2 synthesis by 48% and 81%, respectively. Comparable inhibition was observed on the conversion of exogenous arachidonic acid to PGs by IL-1-stimulated cells, suggesting IL-4 down regulates PG endoperoxide synthase activity. These results demonstrated that IL-4 is antagonistic to inflammatory cytokines upon PG synthesis as well as anti-mitogenic with MC.

本文言語English
ページ(範囲)486-493
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
197
2
DOI
出版ステータスPublished - 1993 12月 15
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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