Intestinal Lymphatic Endothelial Cells Produce R-Spondin3

Reiki Ogasawara, Daigo Hashimoto, Shunsuke Kimura, Eiko Hayase, Takahide Ara, Shuichiro Takahashi, Hiroyuki Ohigashi, Kosuke Yoshioka, Takahiro Tateno, Emi Yokoyama, Ko Ebata, Takeshi Kondo, Junichi Sugita, Masahiro Onozawa, Toshihiko Iwanaga, Takanori Teshima

研究成果: Article

1 引用 (Scopus)

抄録

The R-Spondin (R-Spo) family regulates WNT signaling and stimulates the proliferation and differentiation of intestinal stem cells (ISCs). R-Spo plays a critical role in maintaining intestinal homeostasis, but endogenous producers of R-Spo in the intestine remain to be investigated. We found that R-Spo3 was the major R-Spo family member produced in the intestine and it was predominantly produced by CD45-CD90+CD31+ lymphatic endothelial cells (LECs) in the lamina propria of the intestinal mucosa. Transcriptome analysis demonstrated that LECs highly expressed R-Spo receptor, Lgr5, suggesting an autocrine stimulatory loop in LECs. LECs were significantly reduced in number, and their R-Spo3 production was impaired in intestinal graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation. The impaired production of R-Spo3 in the intestine may be a novel mechanism of delayed tissue repair and defective mucosal defense in intestinal GVHD. We demonstrate a novel role of intestinal LECs in producing R-Spondin3 to maintain intestinal homeostasis.

元の言語English
記事番号10719
ジャーナルScientific reports
8
発行部数1
DOI
出版物ステータスPublished - 2018 12 1
外部発表Yes

Fingerprint

Endothelial Cells
Intestines
Graft vs Host Disease
Homeostasis
Hematopoietic Stem Cell Transplantation
Gene Expression Profiling
Intestinal Mucosa
Mucous Membrane
Stem Cells

ASJC Scopus subject areas

  • General

これを引用

Ogasawara, R., Hashimoto, D., Kimura, S., Hayase, E., Ara, T., Takahashi, S., ... Teshima, T. (2018). Intestinal Lymphatic Endothelial Cells Produce R-Spondin3. Scientific reports, 8(1), [10719]. https://doi.org/10.1038/s41598-018-29100-7

Intestinal Lymphatic Endothelial Cells Produce R-Spondin3. / Ogasawara, Reiki; Hashimoto, Daigo; Kimura, Shunsuke; Hayase, Eiko; Ara, Takahide; Takahashi, Shuichiro; Ohigashi, Hiroyuki; Yoshioka, Kosuke; Tateno, Takahiro; Yokoyama, Emi; Ebata, Ko; Kondo, Takeshi; Sugita, Junichi; Onozawa, Masahiro; Iwanaga, Toshihiko; Teshima, Takanori.

:: Scientific reports, 巻 8, 番号 1, 10719, 01.12.2018.

研究成果: Article

Ogasawara, R, Hashimoto, D, Kimura, S, Hayase, E, Ara, T, Takahashi, S, Ohigashi, H, Yoshioka, K, Tateno, T, Yokoyama, E, Ebata, K, Kondo, T, Sugita, J, Onozawa, M, Iwanaga, T & Teshima, T 2018, 'Intestinal Lymphatic Endothelial Cells Produce R-Spondin3', Scientific reports, 巻. 8, 番号 1, 10719. https://doi.org/10.1038/s41598-018-29100-7
Ogasawara R, Hashimoto D, Kimura S, Hayase E, Ara T, Takahashi S その他. Intestinal Lymphatic Endothelial Cells Produce R-Spondin3. Scientific reports. 2018 12 1;8(1). 10719. https://doi.org/10.1038/s41598-018-29100-7
Ogasawara, Reiki ; Hashimoto, Daigo ; Kimura, Shunsuke ; Hayase, Eiko ; Ara, Takahide ; Takahashi, Shuichiro ; Ohigashi, Hiroyuki ; Yoshioka, Kosuke ; Tateno, Takahiro ; Yokoyama, Emi ; Ebata, Ko ; Kondo, Takeshi ; Sugita, Junichi ; Onozawa, Masahiro ; Iwanaga, Toshihiko ; Teshima, Takanori. / Intestinal Lymphatic Endothelial Cells Produce R-Spondin3. :: Scientific reports. 2018 ; 巻 8, 番号 1.
@article{e2989e0c6bc945cab3e799b04eb785b8,
title = "Intestinal Lymphatic Endothelial Cells Produce R-Spondin3",
abstract = "The R-Spondin (R-Spo) family regulates WNT signaling and stimulates the proliferation and differentiation of intestinal stem cells (ISCs). R-Spo plays a critical role in maintaining intestinal homeostasis, but endogenous producers of R-Spo in the intestine remain to be investigated. We found that R-Spo3 was the major R-Spo family member produced in the intestine and it was predominantly produced by CD45-CD90+CD31+ lymphatic endothelial cells (LECs) in the lamina propria of the intestinal mucosa. Transcriptome analysis demonstrated that LECs highly expressed R-Spo receptor, Lgr5, suggesting an autocrine stimulatory loop in LECs. LECs were significantly reduced in number, and their R-Spo3 production was impaired in intestinal graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation. The impaired production of R-Spo3 in the intestine may be a novel mechanism of delayed tissue repair and defective mucosal defense in intestinal GVHD. We demonstrate a novel role of intestinal LECs in producing R-Spondin3 to maintain intestinal homeostasis.",
author = "Reiki Ogasawara and Daigo Hashimoto and Shunsuke Kimura and Eiko Hayase and Takahide Ara and Shuichiro Takahashi and Hiroyuki Ohigashi and Kosuke Yoshioka and Takahiro Tateno and Emi Yokoyama and Ko Ebata and Takeshi Kondo and Junichi Sugita and Masahiro Onozawa and Toshihiko Iwanaga and Takanori Teshima",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41598-018-29100-7",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Intestinal Lymphatic Endothelial Cells Produce R-Spondin3

AU - Ogasawara, Reiki

AU - Hashimoto, Daigo

AU - Kimura, Shunsuke

AU - Hayase, Eiko

AU - Ara, Takahide

AU - Takahashi, Shuichiro

AU - Ohigashi, Hiroyuki

AU - Yoshioka, Kosuke

AU - Tateno, Takahiro

AU - Yokoyama, Emi

AU - Ebata, Ko

AU - Kondo, Takeshi

AU - Sugita, Junichi

AU - Onozawa, Masahiro

AU - Iwanaga, Toshihiko

AU - Teshima, Takanori

PY - 2018/12/1

Y1 - 2018/12/1

N2 - The R-Spondin (R-Spo) family regulates WNT signaling and stimulates the proliferation and differentiation of intestinal stem cells (ISCs). R-Spo plays a critical role in maintaining intestinal homeostasis, but endogenous producers of R-Spo in the intestine remain to be investigated. We found that R-Spo3 was the major R-Spo family member produced in the intestine and it was predominantly produced by CD45-CD90+CD31+ lymphatic endothelial cells (LECs) in the lamina propria of the intestinal mucosa. Transcriptome analysis demonstrated that LECs highly expressed R-Spo receptor, Lgr5, suggesting an autocrine stimulatory loop in LECs. LECs were significantly reduced in number, and their R-Spo3 production was impaired in intestinal graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation. The impaired production of R-Spo3 in the intestine may be a novel mechanism of delayed tissue repair and defective mucosal defense in intestinal GVHD. We demonstrate a novel role of intestinal LECs in producing R-Spondin3 to maintain intestinal homeostasis.

AB - The R-Spondin (R-Spo) family regulates WNT signaling and stimulates the proliferation and differentiation of intestinal stem cells (ISCs). R-Spo plays a critical role in maintaining intestinal homeostasis, but endogenous producers of R-Spo in the intestine remain to be investigated. We found that R-Spo3 was the major R-Spo family member produced in the intestine and it was predominantly produced by CD45-CD90+CD31+ lymphatic endothelial cells (LECs) in the lamina propria of the intestinal mucosa. Transcriptome analysis demonstrated that LECs highly expressed R-Spo receptor, Lgr5, suggesting an autocrine stimulatory loop in LECs. LECs were significantly reduced in number, and their R-Spo3 production was impaired in intestinal graft-versus-host disease (GVHD) after allogeneic hematopoietic stem cell transplantation. The impaired production of R-Spo3 in the intestine may be a novel mechanism of delayed tissue repair and defective mucosal defense in intestinal GVHD. We demonstrate a novel role of intestinal LECs in producing R-Spondin3 to maintain intestinal homeostasis.

UR - http://www.scopus.com/inward/record.url?scp=85050334239&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85050334239&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-29100-7

DO - 10.1038/s41598-018-29100-7

M3 - Article

C2 - 30013036

AN - SCOPUS:85050334239

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 10719

ER -