Intracellular magnesium level determines cell viability in the MPP+ model of Parkinson's disease

Yutaka Shindo, Ryu Yamanaka, Koji Suzuki, Kohji Hotta, Kotaro Oka

研究成果: Article査読

23 被引用数 (Scopus)

抄録

Parkinson's disease (PD) is a neurodegenerative disorder resulting from mitochondrial dysfunction in dopaminergic neurons. Mitochondria are believed to be responsible for cellular Mg2+ homeostasis. Mg2+ is indispensable for maintaining ordinal cellular functions, hence perturbation of the cellular Mg2+ homeostasis may be responsible for the disorders of physiological functions and diseases including PD. However, the changes in intracellular Mg2+ concentration ([Mg2+]i) and the role of Mg2+ in PD have still been obscure. In this study, we investigated [Mg2+]i and its effect on neurodegeneration in the 1-methyl-4-phenylpyridinium (MPP+) model of PD in differentiated PC12 cells. Application of MPP+ induced an increase in [Mg2+]i immediately via two different pathways: Mg2+ release from mitochondria and Mg2+ influx across cell membrane, and the increased [Mg2+]i sustained for more than 16h after MPP+ application. Suppression of Mg2+ influx decreased the viability of the cells exposed to MPP+. The cell viability correlated highly with [Mg2+]i. In the PC12 cells with suppressed Mg2+ influx, ATP concentration decreased and the amount of reactive oxygen species (ROS) increased after an 8h exposure to MPP+. Our results indicate that the increase in [Mg2+]i inhibited cellular ROS generation and maintained ATP production, which resulted in the protection from MPP+ toxicity.

本文言語English
ページ(範囲)3182-3191
ページ数10
ジャーナルBiochimica et Biophysica Acta - Molecular Cell Research
1853
12
DOI
出版ステータスPublished - 2015 12月 1

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

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