Intracerebroventricular administration of C-type natriuretic peptide suppresses food intake via activation of the melanocortin system in mice

Nobuko Yamada-Goto, Goro Katsuura, Ken Ebihara, Megumi Inuzuka, Yukari Ochi, Yui Yamashita, Toru Kusakabe, Akihiro Yasoda, Noriko Satoh-Asahara, Hiroyuki Ariyasu, Kiminori Hosoda, Kazuwa Nakao

研究成果: Article査読

25 被引用数 (Scopus)

抄録

C-type natriuretic peptide (CNP) and its receptor are abundantly distributed in the brain, especially in the arcuate nucleus (ARC) of the hypothalamus associated with regulating energy homeostasis. To elucidate the possible involvement of CNP in energy regulation, we examined the effects of intracerebroventricular administration of CNP on food intake in mice. The intracerebroventricular administration of CNP-22 and CNP-53 significantly suppressed food intake on 4-h refeeding after 48-h fasting. Next, intracerebroventricular administration of CNP-22 and CNP-53 significantly decreased nocturnal food intake. The increment of food intake induced by neuropeptide Y and ghrelin was markedly suppressed by intracerebroventricular administration of CNP-22 and CNP-53. When SHU9119, an antagonist for melanocortin-3 and melanocortin-4 receptors, was coadministered with CNP-53, the suppressive effect of CNP-53 on refeeding after 48-h fasting was significantly attenuated by SHU9119. Immunohistochemical analysis revealed that intracerebroventricular administration of CNP-53 markedly increased the number of c-Fos - positive cells in the ARC, paraventricular nucleus, dorsomedial hypothalamus, ventromedial hypothalamic nucleus, and lateral hypothalamus. In particular, c-Fos-positive cells in the ARC after intracerebroventricular administration of CNP-53 were coexpressed with α-melanocyte-stimulating hormone immunoreactivity. These results indicated that intracerebroventricular administration of CNP induces an anorexigenic action, in part, via activation of the melanocortin system.

本文言語English
ページ(範囲)1500-1504
ページ数5
ジャーナルDiabetes
62
5
DOI
出版ステータスPublished - 2013 5

ASJC Scopus subject areas

  • 内科学
  • 内分泌学、糖尿病および代謝内科学

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