Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level

R. Kawano; Y. Tsuji; M. Hirano; T. Osaki; K. Kamiya; N. Miki; T. Ide; S. Takeuchi

doi:10.1109/MEMSYS.2013.6474175

Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level

R. Kawano, Y. Tsuji, M. Hirano, T. Osaki, K. Kamiya, N. Miki, T. Ide, S. Takeuchi

機械工学科

研究成果: Conference contribution

抄録

This paper describes an investigation of drug effects for ion channels with recognizing the intra/extracellular directions. We have previously reported the ion channel recordings with bilayer lipid membranes (BLMs) which formed on a parylene micropore using droplets contact method. However, the low reconstitution probability of ion channels into BLMs has been a major issue. In this study, BLMs area and position are regulated with changing the numbers and positions of the parylene micropores. As a result, the simultaneous single channel recordings of K⁺ channel expressed in nerve system with BLM array were able to be achieved at the high probability.

本文言語	English
ホスト出版物のタイトル	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
ページ	57-58
ページ数	2
DOI	https://doi.org/10.1109/MEMSYS.2013.6474175
出版ステータス	Published - 2013
イベント	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 - Taipei, Taiwan, Province of China 継続期間: 2013 1月 20 → 2013 1月 24

出版物シリーズ

名前	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN（印刷版）	1084-6999

Other

Other	IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
国/地域	Taiwan, Province of China
City	Taipei
Period	13/1/20 → 13/1/24

ASJC Scopus subject areas

電子材料、光学材料、および磁性材料
凝縮系物理学
機械工学
電子工学および電気工学

文献へのアクセス

10.1109/MEMSYS.2013.6474175

他のファイルとリンク

引用スタイル

Kawano, R., Tsuji, Y., Hirano, M., Osaki, T., Kamiya, K., Miki, N., Ide, T., & Takeuchi, S. (2013). Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 (pp. 57-58). [6474175] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2013.6474175

Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level. / Kawano, R.; Tsuji, Y.; Hirano, M. その他.
IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. p. 57-58 6474175 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

研究成果: Conference contribution

Kawano, R, Tsuji, Y, Hirano, M, Osaki, T, Kamiya, K, Miki, N, Ide, T & Takeuchi, S 2013, Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level. in IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013., 6474175, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 57-58, IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013, Taipei, Taiwan, Province of China, 13/1/20. https://doi.org/10.1109/MEMSYS.2013.6474175

Kawano R, Tsuji Y, Hirano M, Osaki T, Kamiya K, Miki N その他. Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level. In IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013. 2013. p. 57-58. 6474175. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). doi: 10.1109/MEMSYS.2013.6474175

@inproceedings{5867d417128246a48de3bb9316a6941f,

title = "Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level",

abstract = "This paper describes an investigation of drug effects for ion channels with recognizing the intra/extracellular directions. We have previously reported the ion channel recordings with bilayer lipid membranes (BLMs) which formed on a parylene micropore using droplets contact method. However, the low reconstitution probability of ion channels into BLMs has been a major issue. In this study, BLMs area and position are regulated with changing the numbers and positions of the parylene micropores. As a result, the simultaneous single channel recordings of K+ channel expressed in nerve system with BLM array were able to be achieved at the high probability.",

author = "R. Kawano and Y. Tsuji and M. Hirano and T. Osaki and K. Kamiya and N. Miki and T. Ide and S. Takeuchi",

year = "2013",

doi = "10.1109/MEMSYS.2013.6474175",

language = "English",

isbn = "9781467356558",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "57--58",

booktitle = "IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013",

note = "IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013 ; Conference date: 20-01-2013 Through 24-01-2013",

}

TY - GEN

T1 - Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level

AU - Kawano, R.

AU - Tsuji, Y.

AU - Hirano, M.

AU - Osaki, T.

AU - Kamiya, K.

AU - Miki, N.

AU - Ide, T.

AU - Takeuchi, S.

PY - 2013

Y1 - 2013

N2 - This paper describes an investigation of drug effects for ion channels with recognizing the intra/extracellular directions. We have previously reported the ion channel recordings with bilayer lipid membranes (BLMs) which formed on a parylene micropore using droplets contact method. However, the low reconstitution probability of ion channels into BLMs has been a major issue. In this study, BLMs area and position are regulated with changing the numbers and positions of the parylene micropores. As a result, the simultaneous single channel recordings of K+ channel expressed in nerve system with BLM array were able to be achieved at the high probability.

AB - This paper describes an investigation of drug effects for ion channels with recognizing the intra/extracellular directions. We have previously reported the ion channel recordings with bilayer lipid membranes (BLMs) which formed on a parylene micropore using droplets contact method. However, the low reconstitution probability of ion channels into BLMs has been a major issue. In this study, BLMs area and position are regulated with changing the numbers and positions of the parylene micropores. As a result, the simultaneous single channel recordings of K+ channel expressed in nerve system with BLM array were able to be achieved at the high probability.

UR - http://www.scopus.com/inward/record.url?scp=84875434880&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875434880&partnerID=8YFLogxK

U2 - 10.1109/MEMSYS.2013.6474175

DO - 10.1109/MEMSYS.2013.6474175

M3 - Conference contribution

AN - SCOPUS:84875434880

SN - 9781467356558

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 57

EP - 58

BT - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013

T2 - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013

Y2 - 20 January 2013 through 24 January 2013

ER -

Intra/extracellular investigation for ion channels with lipid bilayer array at the single molecule level

抄録

出版物シリーズ

Other

ASJC Scopus subject areas

文献へのアクセス

他のファイルとリンク

フィンガープリント

引用スタイル