TY - JOUR
T1 - Intratracheal instillation of perfluorocarbon rescued mice with primary pulmonary hypoplasia
AU - Mori, Kazuhiro
AU - Kurihara, Nobuyoshi
AU - Obi, Shinnosuke
AU - Hayashida, Shinya
AU - Tokieda, Keisuke
AU - Ikeda, Kazushige
PY - 2002
Y1 - 2002
N2 - The aim of this study was to investigate the effect of perfluorocarbon on the respiratory status of newborn mice with pulmonary hypoplasia without diaphragmatic defects, following intrauterine exposure to nitrofen. Three groups of newborn mice were compared: pups exposed to nitrofen antenatally without (group A) or with (group B) perfluorocarbon treatment and pups not exposed to nitrofen as a control (group C). Respiratory evaluation was performed by scoring, pressure-volume analysis, and histological examination. At 40 minutes after birth, the survival rates in groups A, B, and C were 51%, 94%, and 95%, respectively. The clinical scores of group B mice at 40 minutes were significantly better than those of group A mice in which pulmonary hypoplasia was induced. In group B, the hysteresis ratio was significantly higher than that in group A, and lung histology showed a significant increase in airspace. An immunohistochemical examination showed that perfluorocarbon did not alter the expression of mature surfactant protein B and surfactant proprotein C. This study demonstrated that treatment with perfluorocarbon was useful in stabilizing critically ill mice with primary pulmonary hypoptasia during the early phase of therapy.
AB - The aim of this study was to investigate the effect of perfluorocarbon on the respiratory status of newborn mice with pulmonary hypoplasia without diaphragmatic defects, following intrauterine exposure to nitrofen. Three groups of newborn mice were compared: pups exposed to nitrofen antenatally without (group A) or with (group B) perfluorocarbon treatment and pups not exposed to nitrofen as a control (group C). Respiratory evaluation was performed by scoring, pressure-volume analysis, and histological examination. At 40 minutes after birth, the survival rates in groups A, B, and C were 51%, 94%, and 95%, respectively. The clinical scores of group B mice at 40 minutes were significantly better than those of group A mice in which pulmonary hypoplasia was induced. In group B, the hysteresis ratio was significantly higher than that in group A, and lung histology showed a significant increase in airspace. An immunohistochemical examination showed that perfluorocarbon did not alter the expression of mature surfactant protein B and surfactant proprotein C. This study demonstrated that treatment with perfluorocarbon was useful in stabilizing critically ill mice with primary pulmonary hypoptasia during the early phase of therapy.
KW - Nitrofen
KW - Partial liquid ventilation
KW - Perfluorocarbon
KW - Pulmonary hypoplasia
KW - Surfactant protein
UR - http://www.scopus.com/inward/record.url?scp=0036347938&partnerID=8YFLogxK
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U2 - 10.1080/01902140290096773
DO - 10.1080/01902140290096773
M3 - Article
C2 - 12217217
AN - SCOPUS:0036347938
VL - 28
SP - 523
EP - 533
JO - Experimental Lung Research
JF - Experimental Lung Research
SN - 0190-2148
IS - 6
ER -