Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation

Naoshi Kawamura; Masaru Udagawa; Tomonobu Fujita; Toshiharu Sakurai; Tomonori Yaguchi; Yutaka Kawakami

doi:10.1007/978-1-4939-0345-0_13

Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation

Naoshi Kawamura, Masaru Udagawa, Tomonobu Fujita, Toshiharu Sakurai, Tomonori Yaguchi, Yutaka Kawakami

先端医科学研究所(細胞)

研究成果: Article › 査読

1 被引用数 (Scopus)

抄録

Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.

本文言語	English
ページ（範囲）	145-153
ページ数	9
ジャーナル	Methods in Molecular Biology
巻	1139
DOI	https://doi.org/10.1007/978-1-4939-0345-0_13
出版ステータス	Published - 2014

ASJC Scopus subject areas

分子生物学
遺伝学

UN SDG

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10.1007/978-1-4939-0345-0_13

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title = "Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation",

abstract = "Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.",

keywords = "BCG-CWS, Cryoablation, Dendritic cells, Immunotherapy, KLH",

author = "Naoshi Kawamura and Masaru Udagawa and Tomonobu Fujita and Toshiharu Sakurai and Tomonori Yaguchi and Yutaka Kawakami",

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AU - Kawamura, Naoshi

AU - Udagawa, Masaru

AU - Fujita, Tomonobu

AU - Sakurai, Toshiharu

AU - Yaguchi, Tomonori

AU - Kawakami, Yutaka

N1 - Publisher Copyright: © Springer Science+Business Media New York 2014.

PY - 2014

Y1 - 2014

N2 - Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.

AB - Intratumoral administration of dendritic cells (DC) following cryoablation of tumor is one of the personalized cancer immunotherapies which is able to induce immune responses to multiple endogenous tumor antigens, including shared and unique antigens. Here we describe protocols of cryoablation of tumors, generation of cultured DC, pretreatment of DC with a Toll-like receptor (TLR)-stimulating purified component of Bacillus Calmette-Guerin cell wall fraction (BCG-CWS) and highly immunogenic keyhole limpet hemocyanin (KLH) antigen, and combined use of tumor cryoablation and intratumoral administration of BCG-CWS-pretreated DC in both a murine model and cancer patients.

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KW - Cryoablation

KW - Dendritic cells

KW - Immunotherapy

KW - KLH

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Intratumoral injection of BCG-CWS-pretreated dendritic cells following tumor cryoablation

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ASJC Scopus subject areas

UN SDG

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