Intricate recognition of glycolipid-like compounds by HIV-1 envelope proteins evaluated with surface plasmon resonance imaging

Tomoko Okada, Arisa Kimura, Hiroshi Miura, Toshinori Nishiyama, Masako Mori, Jyunya Suzuki, Masayo Ogiso, Koji Matsuoka, Toshinori Sato, Kenichi Hatanaka, Norihiko Minoura

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Fusion of human immunodeficiency virus (HIV) to the cell membrane occurs by the specific binding of an envelope protein of HIV-1 (gp120 and gp160) and a glycosphingolipid of the cell membrane. In this study, quantitative and array-based affinity evaluation of gp120 and gp160 was performed by surface plasmon resonance (SPR) and the SPR imaging technique using a substrate immobilized with glycolipid-like compounds (Gb3, GM3, and Lac). Quantitative affinity evaluation showed that gp160 specifically bound to Gb3 and Lac compared with GM3, whereas gp120 showed lower binding affinity and specificity. Array-based evaluation showed that gp160 binds to Gb3 more favorably than Lac and GM3.

本文言語English
ページ(範囲)584-592
ページ数9
ジャーナルJournal of Carbohydrate Chemistry
31
7
DOI
出版ステータスPublished - 2012 9 1

ASJC Scopus subject areas

  • 生化学
  • 有機化学

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