Involvement of autocrine CXCL12/CXCR4 system in the regulation of ovarian carcinoma cell invasion

Nami Miyanishi, Yukino Suzuki, Siro Simizu, Yoshiko Kuwabara, Kouji Banno, Kazuo Umezawa

研究成果: Article査読

36 被引用数 (Scopus)

抄録

Ovarian carcinomas are often highly invasive, especially in the peritoneal cavity; however, the mechanism involved in invasion is not yet fully understood. In the present research, we studied the role of NF-κB in the invasiveness of ovarian carcinoma cells by using (-)-DHMEQ, a specific inhibitor of NF-κB. (-)-DHMEQ inhibited invasion in vitro and the expression of CXCL12 and CXCR4. We found that neutralizing antibody against CXCR4 or knockdown of CXCR4 suppressed the invasion. Proteomic analysis revealed that CXCR4-siRNA treatment lowered the secretion of several invasion-related proteins, such as MMP-9 and uPA. These data imply that (-)-DHMEQ suppressed ovarian cell invasion via inhibition of the NF-κB-regulated autocrine system of CXCL12-CXCR4.

本文言語English
ページ(範囲)154-159
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
403
1
DOI
出版ステータスPublished - 2010 12 3

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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