Involvement of c-Src in carcinoma cell motility and metastasis

M. Sakamoto, M. Takamura, Y. Ino, A. Miura, T. Genda, S. Hirohashi

研究成果: Article査読

26 被引用数 (Scopus)

抄録

Carcinoma cells exhibit dysfunction/dysregulation of cell adhesion systems that correlates with their abilities to migrate, invade, and metastasize. Here we show that the tyrosine kinase c-Src is required for motility and metastasis of two carcinoma cell lines. Adherent KYN-2 cells having a high level of c-Src kinase activity become scattered, extend lamellipodia, and exhibit high motility. Expression of a dominant-negative mutant form of c-Src caused formation of stress fibers and focal adhesions, and markedly reduced motility. HCT15 cells extended lamellipodia and became scattered in response to lysophosphatidic acid stimulation in parallel with transient activation of c-Src, which was inhibited by expression of a dominant-negative mutant form of c-Src or treatment with a specific Src kinase inhibitor. Furthermore, implantation of dominant-negative c-Src transfectants into the peritoneal cavity of SCID mice resulted in reduced peritoneal dissemination compared with control transfectants. These findings indicate that c-Src activation is critically involved in carcinoma cell migration and metastasis.

本文言語English
ページ(範囲)941-946
ページ数6
ジャーナルJapanese Journal of Cancer Research
92
9
DOI
出版ステータスPublished - 2001
外部発表はい

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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