Involvement of histone acetylation in ovarian steroid-induced decidualization of human endometrial stromal cells

Nozomi Sakai, Tetsuo Maruyama, Rei Sakurai, Hirotaka Masuda, Yurie Yamamoto, Aki Shimizu, Ikuko Kishi, Hironori Asada, Satoshi Yamagoe, Yasunori Yoshimura

研究成果: Article

52 引用 (Scopus)

抄録

Histone acetyltransferases and histone deacetylases (HDACs) determine the acetylation status of histones, regulating gene transcription. Decidualization is the progestin-induced differentiation of estrogen-primed endometrial stromal cells (ESCs), which is crucial for implantation and maintenance of pregnancy. We here show that trichostatin A (TSA), a specific HDAC inhibitor, enhances the up-regulation of decidualization markers such as insulin-like growth factor binding protein-1 (IGFBP-1) and prolactin in a dose-dependent manner that is directed by 17β-estradiol (E2) plus progesterone (P4) in cultured ESCs, but not glandular cells, both isolated from human endometrium. Morphological changes resembling decidual transformation were also augmented by co-addition of TSA. Acid urea triton gel analysis and immunoblot using acetylated histone type-specific antibodies demonstrated that treatment with E2 plus P4 significantly increased the levels of acetylated H3 and H4 whose increment was augmented by cotreatment with TSA. Chromatin immunoprecipitation assay revealed that treatment with E2 plus P4 increased the amount of proximal progesterone-responsive region of IGFBP-1 promoter associated with acetylated H4, which was dramatically enhanced by co-addition of TSA. Taken together, our results suggest that histone acetylation is deeply involved in differentiation of human ESCs and that TSA has a potential as an enhancer of decidualization through promotion of progesterone action.

元の言語English
ページ(範囲)16675-16682
ページ数8
ジャーナルJournal of Biological Chemistry
278
発行部数19
DOI
出版物ステータスPublished - 2003 5 9

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trichostatin A
Acetylation
Stromal Cells
Histones
Steroids
Progesterone
Insulin-Like Growth Factor Binding Protein 1
Histone Deacetylases
Pregnancy Maintenance
Histone Acetyltransferases
Chromatin Immunoprecipitation
Progestins
Transcription
Endometrium
Prolactin
Chromatin
Urea
Estradiol
Assays
Estrogens

ASJC Scopus subject areas

  • Biochemistry

これを引用

Involvement of histone acetylation in ovarian steroid-induced decidualization of human endometrial stromal cells. / Sakai, Nozomi; Maruyama, Tetsuo; Sakurai, Rei; Masuda, Hirotaka; Yamamoto, Yurie; Shimizu, Aki; Kishi, Ikuko; Asada, Hironori; Yamagoe, Satoshi; Yoshimura, Yasunori.

:: Journal of Biological Chemistry, 巻 278, 番号 19, 09.05.2003, p. 16675-16682.

研究成果: Article

Sakai, N, Maruyama, T, Sakurai, R, Masuda, H, Yamamoto, Y, Shimizu, A, Kishi, I, Asada, H, Yamagoe, S & Yoshimura, Y 2003, 'Involvement of histone acetylation in ovarian steroid-induced decidualization of human endometrial stromal cells', Journal of Biological Chemistry, 巻. 278, 番号 19, pp. 16675-16682. https://doi.org/10.1074/jbc.M211715200
Sakai, Nozomi ; Maruyama, Tetsuo ; Sakurai, Rei ; Masuda, Hirotaka ; Yamamoto, Yurie ; Shimizu, Aki ; Kishi, Ikuko ; Asada, Hironori ; Yamagoe, Satoshi ; Yoshimura, Yasunori. / Involvement of histone acetylation in ovarian steroid-induced decidualization of human endometrial stromal cells. :: Journal of Biological Chemistry. 2003 ; 巻 278, 番号 19. pp. 16675-16682.
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