Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development

Tomoko Taguchi, Hisami Takenouchi, Jun Matsui, Wei Ran Tang, Mitsuko Itagaki, Yusuke Shiozawa, Kyoko Suzuki, Sachi Sakaguchi, Yohko U. Ktagiri, Takao Takahashi, Hajime Okita, Junichiro Fujimoto, Nobutaka Kiyokawa

研究成果: Article

25 引用 (Scopus)

抄録

Objective. Insulin-like growth factor (IGF)-binding proteins (IGFBPs) are a family of proteins thought to modulate IGF function. By employing an in vitro culture system of human hematopoietic stem cells cocultured with murine bone marrow stromal cells, we examined the effects of IGF-I and IGFBPs on early B-cell development. Materials and Methods. Human CD34+ bone marrow cells were cocultured with murine stromal MS-5 cells for 4 weeks, and pro-B-cell number was analyzed by flow cytometry. After administration of reagents that are supposed to modulate IGF-I or IGFBP function to the culture, the effect on pro-B-cell development was examined. Results. After cultivation for 4 weeks, effective induction of pro-B-cell proliferation was observed. Experiments using several distinct factors, all of which neutralize IGF-I function, revealed that impairment of IGF-I function results in a significant reduction in pro-B-cell development from CD34+ cells. In addition, when the effect of recombinant proteins of IGFBPs and antibodies against IGFBPs were tested, IGFBP-3 was found to inhibit pro-B-cell development, while IGFBP-6 was required for pro-B-cell development. Conclusions. IGF-I is essential for development of bone marrow CD34+ cells into pro-B cells. Moreover, IGFBPs are likely involved in regulation of pro-B-cell development.

元の言語English
ページ(範囲)508-518
ページ数11
ジャーナルExperimental Hematology
34
発行部数4
DOI
出版物ステータスPublished - 2006 4

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Insulin-Like Growth Factor Binding Proteins
B-Lymphoid Precursor Cells
Insulin-Like Growth Factor I
Bone Marrow Cells
Insulin-Like Growth Factor Binding Protein 6
Insulin-Like Growth Factor Binding Protein 3
Somatomedins
Hematopoietic Stem Cells
Mesenchymal Stromal Cells
Recombinant Proteins
Protein Binding
Flow Cytometry
B-Lymphocytes
Cell Count
Cell Proliferation
Antibodies

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

これを引用

Taguchi, T., Takenouchi, H., Matsui, J., Tang, W. R., Itagaki, M., Shiozawa, Y., ... Kiyokawa, N. (2006). Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development. Experimental Hematology, 34(4), 508-518. https://doi.org/10.1016/j.exphem.2006.01.009

Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development. / Taguchi, Tomoko; Takenouchi, Hisami; Matsui, Jun; Tang, Wei Ran; Itagaki, Mitsuko; Shiozawa, Yusuke; Suzuki, Kyoko; Sakaguchi, Sachi; Ktagiri, Yohko U.; Takahashi, Takao; Okita, Hajime; Fujimoto, Junichiro; Kiyokawa, Nobutaka.

:: Experimental Hematology, 巻 34, 番号 4, 04.2006, p. 508-518.

研究成果: Article

Taguchi, T, Takenouchi, H, Matsui, J, Tang, WR, Itagaki, M, Shiozawa, Y, Suzuki, K, Sakaguchi, S, Ktagiri, YU, Takahashi, T, Okita, H, Fujimoto, J & Kiyokawa, N 2006, 'Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development', Experimental Hematology, 巻. 34, 番号 4, pp. 508-518. https://doi.org/10.1016/j.exphem.2006.01.009
Taguchi, Tomoko ; Takenouchi, Hisami ; Matsui, Jun ; Tang, Wei Ran ; Itagaki, Mitsuko ; Shiozawa, Yusuke ; Suzuki, Kyoko ; Sakaguchi, Sachi ; Ktagiri, Yohko U. ; Takahashi, Takao ; Okita, Hajime ; Fujimoto, Junichiro ; Kiyokawa, Nobutaka. / Involvement of insulin-like growth factor-I and insulin-like growth factor binding proteins in pro-B-cell development. :: Experimental Hematology. 2006 ; 巻 34, 番号 4. pp. 508-518.
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abstract = "Objective. Insulin-like growth factor (IGF)-binding proteins (IGFBPs) are a family of proteins thought to modulate IGF function. By employing an in vitro culture system of human hematopoietic stem cells cocultured with murine bone marrow stromal cells, we examined the effects of IGF-I and IGFBPs on early B-cell development. Materials and Methods. Human CD34+ bone marrow cells were cocultured with murine stromal MS-5 cells for 4 weeks, and pro-B-cell number was analyzed by flow cytometry. After administration of reagents that are supposed to modulate IGF-I or IGFBP function to the culture, the effect on pro-B-cell development was examined. Results. After cultivation for 4 weeks, effective induction of pro-B-cell proliferation was observed. Experiments using several distinct factors, all of which neutralize IGF-I function, revealed that impairment of IGF-I function results in a significant reduction in pro-B-cell development from CD34+ cells. In addition, when the effect of recombinant proteins of IGFBPs and antibodies against IGFBPs were tested, IGFBP-3 was found to inhibit pro-B-cell development, while IGFBP-6 was required for pro-B-cell development. Conclusions. IGF-I is essential for development of bone marrow CD34+ cells into pro-B cells. Moreover, IGFBPs are likely involved in regulation of pro-B-cell development.",
author = "Tomoko Taguchi and Hisami Takenouchi and Jun Matsui and Tang, {Wei Ran} and Mitsuko Itagaki and Yusuke Shiozawa and Kyoko Suzuki and Sachi Sakaguchi and Ktagiri, {Yohko U.} and Takao Takahashi and Hajime Okita and Junichiro Fujimoto and Nobutaka Kiyokawa",
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AU - Taguchi, Tomoko

AU - Takenouchi, Hisami

AU - Matsui, Jun

AU - Tang, Wei Ran

AU - Itagaki, Mitsuko

AU - Shiozawa, Yusuke

AU - Suzuki, Kyoko

AU - Sakaguchi, Sachi

AU - Ktagiri, Yohko U.

AU - Takahashi, Takao

AU - Okita, Hajime

AU - Fujimoto, Junichiro

AU - Kiyokawa, Nobutaka

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N2 - Objective. Insulin-like growth factor (IGF)-binding proteins (IGFBPs) are a family of proteins thought to modulate IGF function. By employing an in vitro culture system of human hematopoietic stem cells cocultured with murine bone marrow stromal cells, we examined the effects of IGF-I and IGFBPs on early B-cell development. Materials and Methods. Human CD34+ bone marrow cells were cocultured with murine stromal MS-5 cells for 4 weeks, and pro-B-cell number was analyzed by flow cytometry. After administration of reagents that are supposed to modulate IGF-I or IGFBP function to the culture, the effect on pro-B-cell development was examined. Results. After cultivation for 4 weeks, effective induction of pro-B-cell proliferation was observed. Experiments using several distinct factors, all of which neutralize IGF-I function, revealed that impairment of IGF-I function results in a significant reduction in pro-B-cell development from CD34+ cells. In addition, when the effect of recombinant proteins of IGFBPs and antibodies against IGFBPs were tested, IGFBP-3 was found to inhibit pro-B-cell development, while IGFBP-6 was required for pro-B-cell development. Conclusions. IGF-I is essential for development of bone marrow CD34+ cells into pro-B cells. Moreover, IGFBPs are likely involved in regulation of pro-B-cell development.

AB - Objective. Insulin-like growth factor (IGF)-binding proteins (IGFBPs) are a family of proteins thought to modulate IGF function. By employing an in vitro culture system of human hematopoietic stem cells cocultured with murine bone marrow stromal cells, we examined the effects of IGF-I and IGFBPs on early B-cell development. Materials and Methods. Human CD34+ bone marrow cells were cocultured with murine stromal MS-5 cells for 4 weeks, and pro-B-cell number was analyzed by flow cytometry. After administration of reagents that are supposed to modulate IGF-I or IGFBP function to the culture, the effect on pro-B-cell development was examined. Results. After cultivation for 4 weeks, effective induction of pro-B-cell proliferation was observed. Experiments using several distinct factors, all of which neutralize IGF-I function, revealed that impairment of IGF-I function results in a significant reduction in pro-B-cell development from CD34+ cells. In addition, when the effect of recombinant proteins of IGFBPs and antibodies against IGFBPs were tested, IGFBP-3 was found to inhibit pro-B-cell development, while IGFBP-6 was required for pro-B-cell development. Conclusions. IGF-I is essential for development of bone marrow CD34+ cells into pro-B cells. Moreover, IGFBPs are likely involved in regulation of pro-B-cell development.

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