Involvement of nuclear accumulation of heat shock protein 27 in leptomycin B-induced apoptosis in HeLa cells

Ayako Tsuchiya, Etsu Tashiro, Minoru Yoshida, Masaya Imoto

研究成果: Article

6 引用 (Scopus)

抄録

Leptomycin B (LMB) is a Streptomyces metabolite that inhibits the chromosomal region maintenance (CRM)1-dependent nuclear export of proteins. It also induces apoptosis in several types of cancer cells, by a yet undefined mechanism. We used a functional proteomics approach to delineate the pathways and mediators involved in LMB-induced apoptosis in HeLa cells. Using two-dimensional gel electrophoresis, we searched for proteins accumulated in the nuclei of HeLa cells upon LMB treatment. Among such proteins we found prohibitin and Heat shock protein (Hsp)27, identified by peptide mass fingerprinting and mass spectrometry. Immunocytochemistry was carried out to confirm the LMB-induced nuclear accumulation of these two proteins in HeLa cells. Furthermore, we found that the cytoplasmic expression of Hsp27, but not prohibitin, partially inhibited LMB-induced apoptosis, indicating that nuclear sequestration of Hsp27 was, at least in part, involved in the apoptosis.

元の言語English
ページ(範囲)810-816
ページ数7
ジャーナルJournal of Antibiotics
58
発行部数12
DOI
出版物ステータスPublished - 2005 12

Fingerprint

HSP27 Heat-Shock Proteins
HeLa Cells
Apoptosis
Proteins
Peptide Mapping
Cell Nucleus Active Transport
Electrophoresis, Gel, Two-Dimensional
Streptomyces
Nuclear Proteins
Proteomics
Mass Spectrometry
Immunohistochemistry
Maintenance
leptomycin B
Neoplasms

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

これを引用

@article{59e1bafd7fe148a2908cd1b544cc85d9,
title = "Involvement of nuclear accumulation of heat shock protein 27 in leptomycin B-induced apoptosis in HeLa cells",
abstract = "Leptomycin B (LMB) is a Streptomyces metabolite that inhibits the chromosomal region maintenance (CRM)1-dependent nuclear export of proteins. It also induces apoptosis in several types of cancer cells, by a yet undefined mechanism. We used a functional proteomics approach to delineate the pathways and mediators involved in LMB-induced apoptosis in HeLa cells. Using two-dimensional gel electrophoresis, we searched for proteins accumulated in the nuclei of HeLa cells upon LMB treatment. Among such proteins we found prohibitin and Heat shock protein (Hsp)27, identified by peptide mass fingerprinting and mass spectrometry. Immunocytochemistry was carried out to confirm the LMB-induced nuclear accumulation of these two proteins in HeLa cells. Furthermore, we found that the cytoplasmic expression of Hsp27, but not prohibitin, partially inhibited LMB-induced apoptosis, indicating that nuclear sequestration of Hsp27 was, at least in part, involved in the apoptosis.",
keywords = "Apoptosis, Chemical proteomics, Hsp27, Leptomycin B",
author = "Ayako Tsuchiya and Etsu Tashiro and Minoru Yoshida and Masaya Imoto",
year = "2005",
month = "12",
doi = "10.1038/ja.2005.108",
language = "English",
volume = "58",
pages = "810--816",
journal = "Journal of Antibiotics",
issn = "0021-8820",
publisher = "Japan Antibiotics Research Association",
number = "12",

}

TY - JOUR

T1 - Involvement of nuclear accumulation of heat shock protein 27 in leptomycin B-induced apoptosis in HeLa cells

AU - Tsuchiya, Ayako

AU - Tashiro, Etsu

AU - Yoshida, Minoru

AU - Imoto, Masaya

PY - 2005/12

Y1 - 2005/12

N2 - Leptomycin B (LMB) is a Streptomyces metabolite that inhibits the chromosomal region maintenance (CRM)1-dependent nuclear export of proteins. It also induces apoptosis in several types of cancer cells, by a yet undefined mechanism. We used a functional proteomics approach to delineate the pathways and mediators involved in LMB-induced apoptosis in HeLa cells. Using two-dimensional gel electrophoresis, we searched for proteins accumulated in the nuclei of HeLa cells upon LMB treatment. Among such proteins we found prohibitin and Heat shock protein (Hsp)27, identified by peptide mass fingerprinting and mass spectrometry. Immunocytochemistry was carried out to confirm the LMB-induced nuclear accumulation of these two proteins in HeLa cells. Furthermore, we found that the cytoplasmic expression of Hsp27, but not prohibitin, partially inhibited LMB-induced apoptosis, indicating that nuclear sequestration of Hsp27 was, at least in part, involved in the apoptosis.

AB - Leptomycin B (LMB) is a Streptomyces metabolite that inhibits the chromosomal region maintenance (CRM)1-dependent nuclear export of proteins. It also induces apoptosis in several types of cancer cells, by a yet undefined mechanism. We used a functional proteomics approach to delineate the pathways and mediators involved in LMB-induced apoptosis in HeLa cells. Using two-dimensional gel electrophoresis, we searched for proteins accumulated in the nuclei of HeLa cells upon LMB treatment. Among such proteins we found prohibitin and Heat shock protein (Hsp)27, identified by peptide mass fingerprinting and mass spectrometry. Immunocytochemistry was carried out to confirm the LMB-induced nuclear accumulation of these two proteins in HeLa cells. Furthermore, we found that the cytoplasmic expression of Hsp27, but not prohibitin, partially inhibited LMB-induced apoptosis, indicating that nuclear sequestration of Hsp27 was, at least in part, involved in the apoptosis.

KW - Apoptosis

KW - Chemical proteomics

KW - Hsp27

KW - Leptomycin B

UR - http://www.scopus.com/inward/record.url?scp=31444454220&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=31444454220&partnerID=8YFLogxK

U2 - 10.1038/ja.2005.108

DO - 10.1038/ja.2005.108

M3 - Article

C2 - 16506698

AN - SCOPUS:31444454220

VL - 58

SP - 810

EP - 816

JO - Journal of Antibiotics

JF - Journal of Antibiotics

SN - 0021-8820

IS - 12

ER -