Isolation and characterization of an Escherichia coli mutant lacking the major serine transporter, and cloning of a serine transporter gene

Wakano Ogawa, Takashi Kayahara, Masaaki Tsuda, Tohru Mizushima, Tomofusa Tsuchiya

研究成果: Article査読

15 被引用数 (Scopus)

抄録

L-Serine as well as L-valine inhibits the growth of Escherichia coli cells, and L-isoleucine releases this growth inhibition. We isolated an E. coli mutant (designated as WAT9) that was able to grow on lactate (or glucose) as a carbon source even in the presence of L-serine, the parent not being able to. Cells of WAT9 were not able to grow on L-serine as a carbon source even if L-isoleucine was present in the culture medium, while the parental cells grew. This mutant was shown to lack the principal L-serine transporter in E. coli, the Na+/serine symporter. This mutant is useful for analysis of the role(s) of the Na+/serine symporter in cell physiology and as a host for the cloning of L-serine transporter gene(s). In fact, we cloned a gene encoding a serine transporter from chromosomal DNA of E. coli using WAT9 as the host. The gene enabled the mutant cells to grow on L-serine. Transport activity for L-serine was restored in the mutant cells harboring a plasmid carrying the gene. We partially sequenced the gene and found that it was the tdcC gene. We showed that TdcC is an H+/serine symporter.

本文言語English
ページ(範囲)1241-1245
ページ数5
ジャーナルJournal of biochemistry
122
6
DOI
出版ステータスPublished - 1997 12月

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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