Isolation and characterization of inhibitory factors of DNA polymerase III holoenzyme from Escherichia coli

Masakazu Hase, Tohru Mizushima, Tsutomu Katayama, Kazuhisa Sekimizu

研究成果: Article

抜粋

We isolated fractions by Mono Q chromatography that inhibited the activity of Escherichia coli DNA polymerase III holoenzyme using an assay system with a primed single-stranded DNA template coated with single-stranded DNA binding protein (SSB). The inhibitory activities were inactivated by heat-treatment at 100 °C for 10 min, suggesting that they are proteins. The factors did not inhibit the activity of RNA polymerase of Escherichia coli. The inhibitory effects were less potent for the activities of the large (Klenow) fragment of DNA polymerase I and T4 DNA polymerase than for DNA polymerase HI holoenzyme. No degradation of single-or double-stranded DNA was observed in the fractions, indicating that inhibition was not due to degradation of the DNA.

元の言語English
ページ(範囲)215-220
ページ数6
ジャーナルFEMS microbiology letters
130
発行部数2-3
DOI
出版物ステータスPublished - 1995 8 1

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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