Isolation and identification of a phytotoxic substance in kiwifruit leaves

S. Okada, Arihiro Iwasaki, I. Kataoka, Kiyotake Suenaga, H. Kato-Noguchi

研究成果: Conference contribution

抄録

The yield of kiwifruit [Actinidia deliciosa (A. Chev.) C.F. Liang et A.R. Ferguson] often decreases after 20 years of cultivation. Replanted young kiwifruit plants cannot grow well in fields where aged trees were previously cultivated. Phytotoxicity is considered one possible reason for this problem. However, allelopathic or phytotoxic substances from kiwifruit have not been reported. This study aimed to isolate and identify potentially allelopathic active substances in kiwifruit leaves. Extracts from kiwifruit leaves inhibited the growth of cress, suggesting that kiwifruit leaf extracts may contain growth-inhibitory substances. The extracts were then purified using a silica-gel column, Sephadex LH-20 column, ODS cartridge, and HPLC. The inhibitory activity of all fractions obtained in each purification step was determined by the cress bioassay. Quercitrin was finally isolated and identified as a growth-inhibitory substance. This substance inhibited cress root growth by more than 50% of control at 3 mM. The aglycone of quercitrin, quercetin, did not significantly inhibit cress growth. Quercitrin may work as an allelopathic substance and cause the kiwifruit replant problem. Further study is in progress to investigate the influence of kiwifruit leaf extracts and these flavonoids on kiwifruit seedlings.

元の言語English
ホスト出版物のタイトル9th International Symposium on Kiwifruit
編集者M.D. Antunes, P.P. Gallego
出版者International Society for Horticultural Science
ページ207-212
ページ数6
ISBN(電子版)9789462612150
出版物ステータスPublished - 2018 10 31

出版物シリーズ

名前Acta Horticulturae
1218
ISSN(印刷物)0567-7572
ISSN(電子版)2406-6168

Fingerprint

kiwifruit
quercitrin
leaves
leaf extracts
Actinidia deliciosa
allelochemicals
extracts
silica gel
phytotoxicity
quercetin
root growth
flavonoids
bioassays
seedlings

ASJC Scopus subject areas

  • Horticulture

これを引用

Okada, S., Iwasaki, A., Kataoka, I., Suenaga, K., & Kato-Noguchi, H. (2018). Isolation and identification of a phytotoxic substance in kiwifruit leaves. : M. D. Antunes, & P. P. Gallego (版), 9th International Symposium on Kiwifruit (pp. 207-212). (Acta Horticulturae; 巻数 1218). International Society for Horticultural Science.

Isolation and identification of a phytotoxic substance in kiwifruit leaves. / Okada, S.; Iwasaki, Arihiro; Kataoka, I.; Suenaga, Kiyotake; Kato-Noguchi, H.

9th International Symposium on Kiwifruit. 版 / M.D. Antunes; P.P. Gallego. International Society for Horticultural Science, 2018. p. 207-212 (Acta Horticulturae; 巻 1218).

研究成果: Conference contribution

Okada, S, Iwasaki, A, Kataoka, I, Suenaga, K & Kato-Noguchi, H 2018, Isolation and identification of a phytotoxic substance in kiwifruit leaves. : MD Antunes & PP Gallego (版), 9th International Symposium on Kiwifruit. Acta Horticulturae, 巻. 1218, International Society for Horticultural Science, pp. 207-212.
Okada S, Iwasaki A, Kataoka I, Suenaga K, Kato-Noguchi H. Isolation and identification of a phytotoxic substance in kiwifruit leaves. : Antunes MD, Gallego PP, 編集者, 9th International Symposium on Kiwifruit. International Society for Horticultural Science. 2018. p. 207-212. (Acta Horticulturae).
Okada, S. ; Iwasaki, Arihiro ; Kataoka, I. ; Suenaga, Kiyotake ; Kato-Noguchi, H. / Isolation and identification of a phytotoxic substance in kiwifruit leaves. 9th International Symposium on Kiwifruit. 編集者 / M.D. Antunes ; P.P. Gallego. International Society for Horticultural Science, 2018. pp. 207-212 (Acta Horticulturae).
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