Isolation and in vitro expansion of human colonic stem cells

Peter Jung, Toshiro Sato, Anna Merlos-Suárez, Francisco M. Barriga, Mar Iglesias, David Rossell, Herbert Auer, Mercedes Gallardo, Maria A. Blasco, Elena Sancho, Hans Clevers, Eduard Batlle

研究成果: Article

382 被引用数 (Scopus)

抄録

Here we describe the isolation of stem cells of the human colonic epithelium. Differential cell surface abundance of ephrin type-B receptor 2 (EPHB2) allows the purification of different cell types from human colon mucosa biopsies. The highest EPHB2 surface levels correspond to epithelial colonic cells with the longest telomeres and elevated expression of intestinal stem cell (ISC) marker genes. Moreover, using culturing conditions that recreate the ISC niche, a substantial proportion of EPHB2-high cells can be expanded in vitro as an undifferentiated and multipotent population.

本文言語English
ページ(範囲)1225-1227
ページ数3
ジャーナルNature medicine
17
10
DOI
出版ステータスPublished - 2011 10 1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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