Isolation and sequence determination of rat cardiac natriuretic peptide

Yoshikazu Kambayashi, Kazuwa Nakao, Hiroshi Itoh, Kiminori Hosoda, Yoshihiko Saito, Takayuki Yamada, Masashi Mukoyama, Hiroshi Arai, Gotaro Shirakami, Shin ichi Suga, Yoshihiro Ogawa, Michihisa Jougasaki, Naoto Minamino, Kenji Kangawa, Hisayuki Matsuo, Ken Inouye, Hiroo Imura

研究成果: Article査読

61 被引用数 (Scopus)

抄録

We have isolated a cardiac natriuretic peptide of 5K daltons from the rat atrium and determined its amino acid sequence. The 5K cardiac natriuretic peptide was elucidated to be a 45-amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-E-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence [51-95] of the rat brain natriuretic peptide (BNP) precursor deduced from the cDNA sequence. The 5K cardiac natriuretic peptide, or BNP[51-95], was identified as the major storage and secretory form derived from the BNP precursor in the rat heart.

本文言語English
ページ(範囲)233-240
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
163
1
DOI
出版ステータスPublished - 1989 8月 30
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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