Isolation and transplantation of dopaminergic neurons generated from mouse embryonic stem cells

Takahito Yoshizaki, Motoki Inaji, Hiroko Kouike, Takuya Shimazaki, Kazunobu Sawamoto, Kiyoshi Ando, Isao Date, Kazuto Kobayashi, Tetsuya Suhara, Yasuo Uchiyama, Hideyuki Okano

研究成果: Article査読

75 被引用数 (Scopus)

抄録

Embryonic stem (ES) cells differentiate into dopamine (DA)-producing neurons when co-cultured with PA6 stromal cells, but the resulting cultures contain a variety of unidentified cells. In order to label live DA neurons in mixed populations, we introduced a GFP reporter under the control of the tyrosine hydroxylase (TH) gene promoter into ES cells. GFP expression was observed in TH-immunoreactive cells that differentiated from the ES cells that carried the TH-GFPreporter gene. DA neurons expressing GFP were sorted from the mixed cell population by fluorescence-activated cell sorting of cells exhibiting GFP fluorescence, and the sorted GFP+ cells obtained were transplanted into a rat model of Parkinson's disease. Some of these cells survived and innervated the host striatum, resulting in a partial recovery from parkinsonian behavioral defects. This strategy of isolation and transplantation of ES-cell-derived DA neurons should be useful for cellular and molecular studies of DA neurons and for clinical application in the treatment of Parkinson's disease.

本文言語English
ページ(範囲)33-37
ページ数5
ジャーナルNeuroscience Letters
363
1
DOI
出版ステータスPublished - 2004 6月 3

ASJC Scopus subject areas

  • 神経科学(全般)

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