Isolation of RNase H genes that are essential for growth of Bacillus subtilis 168

Mitsuhiro Itaya, Akira Omori, Shigenori Kanaya, Robert J. Crouch, Teruo Tanaka, Kanae Kondo

研究成果: Article査読

72 被引用数 (Scopus)

抄録

Two genes encoding functional RNase H (EC 3.1.26.4) were isolated from a gram-positive bacterium, Bacillus subtilis 168. Two DNA clones exhibiting RNase H activities both in vivo and in vitro were obtained from a B. subtilis DNA library. One (28.2 kDa) revealed high similarity to Escherichia coli RNase HII, encoded by the rnhB gene. The other (33.9 kDa) was designated rnhC and encodes B. subtilis RNase HIII. The B. subtilis genome has an rnhA homologue, the product of which has not yet shown RNase H activity. Analyses of all three B. subtilis genes revealed that rnhB and rnhC cannot be simultaneously inactivated. This observation indicated that in B. subtilis both the rnhB and rnhC products are involved in certain essential cellular processes that are different from those suggested by E. coli rnh mutation studies. Sequence conservation between the rnhB and rnhC genes implies that both originated from a single ancestral RNase H gene. The roles of bacterial RNase H may be indicated by the single rnhC homologue in the small genome of Mycoplasma species.

本文言語English
ページ(範囲)2118-2123
ページ数6
ジャーナルJournal of Bacteriology
181
7
DOI
出版ステータスPublished - 1999 4月
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学

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