Jam1a-Jam2a interactions regulate haematopoietic stem cell fate through Notch signalling

Isao Kobayashi, Jingjing Kobayashi-Sun, Albert D. Kim, Claire Pouget, Naonobu Fujita, Toshio Suda, David Traver

研究成果: Article

55 引用 (Scopus)

抄録

Notch signalling plays a key role in the generation of haematopoietic stem cells (HSCs) during vertebrate development1-3 and requires intimate contact between signal-emitting and signal-receiving cells, although little is known regarding when, where and how these intercellular events occur. We previously reported that the somitic Notch ligands, Dlc and Dld, are essential for HSC specification4. It has remained unclear, however, how these somitic requirements are connected to the later emergence of HSCs from the dorsal aorta. Here we show in zebrafish that Notch signalling establishes HSC fate as their shared vascular precursors migrate across the ventral face of the somite and that junctional adhesion molecules (JAMs) mediate this required Notch signal transduction. HSC precursors express jam1a (also known as f11r) and migrate axially across the ventral somite, where Jam2a and the Notch ligands Dlc and Dld are expressed. Despite no alteration in the expression of Notch ligand or receptor genes, loss of function of jam1a led to loss of Notch signalling and loss of HSCs. Enforced activation of Notch in shared vascular precursors rescued HSCs in jam1a or jam2a deficient embryos. Together, these results indicate that Jam1a-Jam2a interactions facilitate the transduction of requisite Notch signals from the somite to the precursors of HSCs, and that these events occur well before formation of the dorsal aorta.

元の言語English
ページ(範囲)319-323
ページ数5
ジャーナルNature
512
発行部数7514
DOI
出版物ステータスPublished - 2014 8 21

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Hematopoietic Stem Cells
Somites
Ligands
Blood Vessels
Aorta
Junctional Adhesion Molecules
Zebrafish
Vertebrates
Signal Transduction
Embryonic Structures
Genes

ASJC Scopus subject areas

  • General

これを引用

Kobayashi, I., Kobayashi-Sun, J., Kim, A. D., Pouget, C., Fujita, N., Suda, T., & Traver, D. (2014). Jam1a-Jam2a interactions regulate haematopoietic stem cell fate through Notch signalling. Nature, 512(7514), 319-323. https://doi.org/10.1038/nature13623

Jam1a-Jam2a interactions regulate haematopoietic stem cell fate through Notch signalling. / Kobayashi, Isao; Kobayashi-Sun, Jingjing; Kim, Albert D.; Pouget, Claire; Fujita, Naonobu; Suda, Toshio; Traver, David.

:: Nature, 巻 512, 番号 7514, 21.08.2014, p. 319-323.

研究成果: Article

Kobayashi, I, Kobayashi-Sun, J, Kim, AD, Pouget, C, Fujita, N, Suda, T & Traver, D 2014, 'Jam1a-Jam2a interactions regulate haematopoietic stem cell fate through Notch signalling', Nature, 巻. 512, 番号 7514, pp. 319-323. https://doi.org/10.1038/nature13623
Kobayashi I, Kobayashi-Sun J, Kim AD, Pouget C, Fujita N, Suda T その他. Jam1a-Jam2a interactions regulate haematopoietic stem cell fate through Notch signalling. Nature. 2014 8 21;512(7514):319-323. https://doi.org/10.1038/nature13623
Kobayashi, Isao ; Kobayashi-Sun, Jingjing ; Kim, Albert D. ; Pouget, Claire ; Fujita, Naonobu ; Suda, Toshio ; Traver, David. / Jam1a-Jam2a interactions regulate haematopoietic stem cell fate through Notch signalling. :: Nature. 2014 ; 巻 512, 番号 7514. pp. 319-323.
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