The (1R,6S)- and (1R,2S,6S)-diastereoisomers of cyclophellitol were found to be effective irreversible inactivators of α-D-glucosidase and α-D-mannosidase, respectively. The (1R,6S)-diastereoisomer inactivates brewers yeast α-D-glucosidase according to pseudo-first order kinetics with inactivation constants of Ki = 26.9 μM, ki = 0.401 min-1 while the (1R,2S,6S)-diastereoisomer inactivates jack beans α-D-mannosidase in a similar manner with Ki = 120 μM, ki = 2.85 min-1. The irreversibility of these compounds was evidenced by the lack of reactivation upon dialysis of the inactivated enzyme.
|ジャーナル||Biochemical and Biophysical Research Communications|
|出版ステータス||Published - 1995 8月 1|
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