Kinetic-Studies on Cyclophellitol Analogs - Mechanism-Based Inactivators

V. W.F. Tai, P. H. Fung, Y. S. Wong, T. K.M. Shing

研究成果: Article査読

7 被引用数 (Scopus)

抄録

The (1R,6S)- and (1R,2S,6S)-diastereoisomers of cyclophellitol were found to be effective irreversible inactivators of α-D-glucosidase and α-D-mannosidase, respectively. The (1R,6S)-diastereoisomer inactivates brewers yeast α-D-glucosidase according to pseudo-first order kinetics with inactivation constants of Ki = 26.9 μM, ki = 0.401 min-1 while the (1R,2S,6S)-diastereoisomer inactivates jack beans α-D-mannosidase in a similar manner with Ki = 120 μM, ki = 2.85 min-1. The irreversibility of these compounds was evidenced by the lack of reactivation upon dialysis of the inactivated enzyme.

本文言語English
ページ(範囲)175-180
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
213
1
DOI
出版ステータスPublished - 1995 8月 1

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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