Kinetics of chromatid break repair in G2-human fibroblasts exposed to low- and high-LET radiations

T. Kawata, M. Durante, K. George, Y. Furusawa, E. Gotoh, N. Takai, H. Wu, F. A. Cucinotta

研究成果: Article査読

3 被引用数 (Scopus)

抄録

The purpose of this study is to determine the kinetics of chromatid break rejoining following exposure to radiations of different quality. Exponentially growing human fibroblast cells AG1522 were irradiated with γ-rays, energetic carbon (290 MeV/u), silicon (490 MeV/u) and iron (200 MeV/u, 600 MeV/u). Chromosomes were prematurely condensed using calyculin A. Prematurely condensed chromosomes were collected after several post-irradiation incubation times, ranging from 5 to 600 minutes, and the number of chromatid breaks and exchanges in G2 cells were scored. The relative biological effectiveness (RBE) for initial chromatid breaks per unit dose showed LET dependency having a peak at 55 keV/μm silicon (2.4) or 80 keV/μm carbon particles (2.4) and then decreased with increasing LET. The kinetics of chromatid break rejoining following low- or high-LET irradiation consisted of two exponential components. Chromatid breaks decreased rapidly after exposure, and then continued to decrease at a slower rate. The rejoining kinetics was similar for exposure to each type of radiation, although the rate of unrejoined breaks was higher for highLET radiation. Chromatid exchanges were also formed quickly.

本文言語English
ページ(範囲)226-228
ページ数3
ジャーナルPhysica Medica
17
SUPPL. 1
出版ステータスPublished - 2001 9 17

ASJC Scopus subject areas

  • 生物理学
  • 放射線学、核医学およびイメージング
  • 物理学および天文学(全般)

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