KRAS mutations in cell-free DNA from preoperative and postoperative sera as a pancreatic cancer marker: A retrospective study

Yutaka Nakano, Minoru Kitago, Sachiko Matsuda, Yuki Nakamura, Yusuke Fujita, Shunichi Imai, Masahiro Shinoda, Hiroshi Yagi, Yuta Abe, Taizo Hibi, Yoko Nishimura, Ayano Takeuchi, Yutaka Endo, Osamu Itano, Yuukou Kitagawa

研究成果: Article

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Background:Pancreatic ductal adenocarcinoma (PDAC) has very poor prognosis despite existing multimodal therapies. This study aimed to investigate whether KRAS mutations at codons 12/13 in cell-free DNA (cfDNA) from preoperative and postoperative sera from patients with PDAC can serve as a predictive biomarker for treatment response and outcomes after surgery.Methods:Preoperative and postoperative serum samples obtained from 45 patients with PDAC whom underwent curative pancreatectomy at our institution between January 2013 and July 2016 were retrospectively analysed. Peptide nucleic acid-directed PCR clamping was used to identify KRAS mutations in cfDNA.Results:Among the 45 patients enrolled, 11 (24.4%) and 20 (44.4%) had KRAS mutations in cfDNA from preoperative and postoperative sera, respectively. Multivariate analysis revealed that KRAS mutations in postoperative serum (hazard ratio (HR)=2.919; 95% confidence interval (CI)=1.109-5.621; P=0.027) are an independent prognostic factor for disease-free survival. Furthermore, the shift from wild-type KRAS in preoperative to mutant KRAS in postoperative cfDNA (HR=9.419; 95% Cl=2.015-44.036; P=0.004) was an independent prognostic factor for overall survival.Conclusions:Changes in KRAS mutation status between preoperative and postoperative cfDNA may be a useful predictive biomarker for survival and treatment response.

元の言語English
ページ(範囲)662-669
ページ数8
ジャーナルBritish Journal of Cancer
118
発行部数5
DOI
出版物ステータスPublished - 2018 3 6

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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