TY - JOUR
T1 - Ktedonoketone and 2’-oxosattabacin, benzenoid metabolites from a thermophilic bacterium Thermosporothrix hazakensis in the phylum Chloroflexi
AU - Igarashi, Yasuhiro
AU - Yamamoto, Kazuki
AU - Ueno, Chiaki
AU - Yamada, Nodoka
AU - Saito, Katsuya
AU - Takahashi, Kazuki
AU - Enomoto, Masaru
AU - Kuwahara, Shigefumi
AU - Oikawa, Tsutomu
AU - Tashiro, Etsu
AU - Imoto, Masaya
AU - Xiaohanyao, Ye
AU - Zhou, Tao
AU - Harunari, Enjuro
AU - Oku, Naoya
PY - 2019/9/1
Y1 - 2019/9/1
N2 - A thermophilic bacterium Thermosporothrix hazakensis NBRC 105916 which belongs to the class Ktedonobacteria was investigated to explore its biosynthetic potential of secondary metabolites. UV-guided fractionation led to the identification of a new benzenoid metabolite designated ktedonoketone (6) and an α-diketone metabolite 2’-oxosattabacin (7) along with five known compounds. Compound 7 was previously described as a synthetic compound, but this is the first finding as a natural product. Compound 7 induced adipocyte differentiation at 10–20 μM and autophagy at 1–10 μM. Compound 6 showed weak inducing activity of adipocyte differentiation. The biosynthetic origin of hazakacin (3), an acyloin-type compound, was elucidated by 13C-labeled precursor-feeding experiments.
AB - A thermophilic bacterium Thermosporothrix hazakensis NBRC 105916 which belongs to the class Ktedonobacteria was investigated to explore its biosynthetic potential of secondary metabolites. UV-guided fractionation led to the identification of a new benzenoid metabolite designated ktedonoketone (6) and an α-diketone metabolite 2’-oxosattabacin (7) along with five known compounds. Compound 7 was previously described as a synthetic compound, but this is the first finding as a natural product. Compound 7 induced adipocyte differentiation at 10–20 μM and autophagy at 1–10 μM. Compound 6 showed weak inducing activity of adipocyte differentiation. The biosynthetic origin of hazakacin (3), an acyloin-type compound, was elucidated by 13C-labeled precursor-feeding experiments.
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U2 - 10.1038/s41429-019-0195-7
DO - 10.1038/s41429-019-0195-7
M3 - Article
C2 - 31164714
AN - SCOPUS:85066940385
VL - 72
SP - 653
EP - 660
JO - Journal of Antibiotics
JF - Journal of Antibiotics
SN - 0021-8820
IS - 9
ER -