Kupffer cell-mediated recruitment of rat dendritic cells to the liver: Roles of N-acetylgalactosamine-specific sugar receptors

Ryosuke Uwatoku, Makoto Suematsu, Taichi Ezaki, Takahito Saiki, Makoto Tsuiji, Tatsuro Irimura, Norifumi Kawada, Tatsuo Suganuma, Makoto Naito, Kenjiro Matsuno

研究成果: Article査読

38 被引用数 (Scopus)

抄録

Background & Aims: Tissue recruitment of dendritic cells (DCs) is essential for antigen presentation. This study aimed to examine cellular and molecular mechanisms for DC recruitment to the liver. Methods: Purified rat DCs were injected into circulation and their traffics were analyzed in normal and Kupffer cell-depleted rats by intravital confocal microscopy and immunohistology. Affinities of DCs to sinusoidal cells were examined by a cell-binding assay. DC precursor recruitment was induced by particulate injection. Results: Both DC precursors and DCs at the antigen-transporting stage could be recruited to the liver, and their majority initially showed a selective binding to Kupffer cells. In the Kupffer cell-depleted rats, DCs could neither be recruited to the liver nor adhere to sinusoidal walls. Pretreatment with varied monosaccharides showed that sugar residues consisting of N-acetylgalactosamine were necessary for this binding. The binding was calcium-dependent, implying the C-type lectin involvement. Furthermore, DCs could endocytose N-acetylgalactosamine polymers in a receptor-specific manner. Conclusions: The DC-Kupffer cell binding through N-acetylgalactosamine-specific C-type lectin-like receptors is crucial for DC recruitment to the, liver. Rat DCs at least partly possess receptors for endocytosis of galactosylated antigens. These DC receptors as well as Kupffer cell lectins are presumably responsible for this binding.

本文言語English
ページ(範囲)1460-1472
ページ数13
ジャーナルGastroenterology
121
6
DOI
出版ステータスPublished - 2001

ASJC Scopus subject areas

  • 肝臓学
  • 消化器病学

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