Labeling embryonic mouse central nervous system cells by in utero electroporation

Hidenori Tabata, Kazunori Nakajima

研究成果: Review article査読

72 被引用数 (Scopus)

抄録

During cerebral development, neurons are generated near the ventricle and then migrate toward the pial surface. In this review, we describe the method of in utero electroporation, this method allows the morphology of the migrating neurons to be visualized and the effect of overexpression or knock down of any gene to be examined. After electroporation of a green fluorescent protein (GFP) expression vector by this method, GFP-positive cells are first found in the ventricular zone, and their distribution then gradually shift toward the pial surface. A few days later, most of the GFP positive cells were aligned beneath the marginal zone, with the normal course of cortical neuronal migration.

本文言語English
ページ(範囲)507-511
ページ数5
ジャーナルDevelopment Growth and Differentiation
50
6
DOI
出版ステータスPublished - 2008 8 1

ASJC Scopus subject areas

  • 発生生物学
  • 細胞生物学

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