Laser capture microdissection and mRNA characterization of mouse airway epithelium: Methodological considerations

Tomoko Betsuyaku, Robert M. Senior

研究成果: Article

18 引用 (Scopus)

抜粋

The use of laser capture microdissection (LCM) to obtain epithelial cells lining the distal airways for gene profiling is described. In the mouse, the distal airways are particularly attractive for LCM as there is very high percentage of a single cell type, Clara cells, lining these airways. It is shown that the RNA from distal airway epithelial cells harvested by LCM is well preserved and that with linear amplification sufficient cRNA for microarray analysis can be attained from small numbers of cells.

元の言語English
ページ(範囲)229-234
ページ数6
ジャーナルMicron
35
発行部数4
DOI
出版物ステータスPublished - 2004 6

ASJC Scopus subject areas

  • Structural Biology
  • Materials Science(all)
  • Physics and Astronomy(all)
  • Cell Biology

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